Abstract
Ototoxic damage caused by aminoglycosides (AG) leads to the loss of cochlear hair cells (HCs). In mammals, mature cochlear HCs are unable to regenerate, and their loss results in permanent hearing deficits. Our objective was to protect the inner ear from damage after an AG challenge. The generation of reactive oxygen species (ROS), one of the earliest events in the process of AG ototoxicity, is considered to play a key role in the initiation of HC death. We examined whether Hangesha-shin-to (TJ-014), a traditional Japanese Kampo medicine considered to be a potent antioxidant, protects HCs from gentamicin (GM)-induced damage. Organ of Corti explants removed from postnatal day 3-5 rats were maintained in tissue culture and exposed to 50μM GM for up to 48h. The effects of TJ-014 on GM-induced ototoxicity were assessed by HC counts and immunohistochemistry against cleaved caspase-3, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and a probe reacting to mitochondrial function changes. TJ-014 treatments significantly reduced GM-induced HC loss and immunoreactivities for cleaved caspase-3 and 8-OHdG; these effects were correlated with increasing TJ-014 concentrations. Moreover, TJ-014 protected the mitochondrial membrane potential from GM otot...Continue Reading
References
May 1, 1996·Hearing Research·B B Song, J Schacht
Oct 1, 1996·Biological & Pharmaceutical Bulletin·Y KaseM Okada
Feb 20, 1998·Japanese Journal of Pharmacology·Y KaseT Kamataki
Mar 30, 1999·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·M TakumidaM Anniko
Feb 17, 2000·Hearing Research·M TakumidaD M Zhang
Jun 14, 2002·Hearing Research·Jukka YlikoskiUlla Pirvola
Mar 6, 2003·Hearing Research·Xiaorui ShiAlfred L Nuttall
Aug 15, 2003·World Journal of Gastroenterology : WJG·Jin-Sheng GuoMarcel Wing Leung Koo
Jul 16, 2005·Cell Death and Differentiation·H JiangJ Schacht
Sep 29, 2006·Hearing Research·Kazuma SugaharaLisa L Cunningham
Sep 8, 2007·Current Opinion in Otolaryngology & Head and Neck Surgery·Mark Douglas Rizzi, Keiko Hirose
Sep 8, 2007·Current Opinion in Otolaryngology & Head and Neck Surgery·Ilaaf DarratMichael D Seidman
Feb 19, 2008·Neuroreport·Hai-Ying LiuWen-Yuan Gao
Mar 26, 2009·Neuroscience·Y H ChoungA F Ryan
Apr 3, 2009·Neuroreport·Yayoi S KikkawaJuichi Ito
Dec 17, 2009·Neuroscience Letters·Tong Ho KangRaekil Park
Aug 27, 2010·Current Medicinal Chemistry·A L PoirrierB Malgrange
Sep 17, 2011·Current Medicinal Chemistry·K TabuchiA Hara
Jun 20, 2014·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Taku YamashitaAkihiro Shiotani
Citations
Oct 3, 2017·Autophagy·Zuhong HeRenjie Chai
Sep 27, 2018·Journal of Cellular and Molecular Medicine·Meijuan ZhouHaibo Wang
Dec 2, 2021·Experimental and Therapeutic Medicine·Wei LiXia Gao