The benPK operon, proposed to play a role in transport, is part of a regulon for benzoate catabolism in Acinetobacter sp. strain ADP1

Microbiology
Todd J ClarkEllen L Neidle

Abstract

BenM and CatM are distinct, but similar, LysR-type transcriptional regulators of the soil bacterium Acinetobacter sp. strain ADP1. Together, the two regulators control the expression of at least 14 genes involved in the degradation of aromatic compounds via the catechol branch of the beta-ketoadipate pathway. In these studies, BenM and CatM were each purified to homogeneity to test the possibility that they regulate the expression of two additional genes, benP and benK, that are adjacent to benM on the chromosome. Each regulator bound to a DNA fragment containing the benP promoter region. Additional transcriptional studies suggested that benP and benK are co-transcribed as an operon, and a site of transcription initiation was identified. Alignment of this initiation site with those of several CatM- and BenM-regulated genes revealed common regulatory motifs. Mutants lacking both CatM and BenM failed to activate benP transcription. The ability of each protein to regulate gene expression was inferred from strains lacking either CatM or BenM that were still capable of increasing benP expression in response to cis,cis-muconate. This compound has previously been shown to induce all enzymes of the catechol branch of the beta-ketoadipa...Continue Reading

References

Oct 1, 1990·Journal of Bacteriology·L A Gregg-Jolly, L N Ornston
Apr 1, 1969·Journal of Bacteriology·E Juni, A Janik
Jan 1, 1993·Annual Review of Microbiology·M A Schell
Jan 1, 1996·Annual Review of Microbiology·C S Harwood, R E Parales
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·E R OliveraJ M Luengo
Mar 27, 1999·The Journal of Biological Chemistry·D M TobiasonA C Glasgow
Jul 27, 1999·Journal of Bacteriology·R M JonesP A Williams
Jun 2, 2000·The Journal of Membrane Biology·M H Saier
Aug 10, 2000·Molecular Microbiology·R KoebnikP Van Gelder

❮ Previous
Next ❯

Citations

Jan 26, 2016·Structure·Michael ZahnBert van den Berg
Jul 25, 2012·Journal of Biotechnology·Tino PolenMichael Bott
Jun 12, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Wenjie GongEdmund Maser
Mar 18, 2009·Letters in Applied Microbiology·E V P PandeetiD Siddavattam
Apr 30, 2009·Molecular Microbiology·Sarah H CravenEllen L Neidle
Feb 13, 2007·Journal of Molecular Biology·Obidimma C EzezikaCory Momany
Nov 28, 2012·Microbial Pathogenesis·Jared D HeffronSusan L Welkos
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Becky M BundyEllen L Neidle
May 9, 2012·Antimicrobial Agents and Chemotherapy·Manuella Catel-FerreiraEmmanuelle Dé
Jul 17, 2012·Journal of Bacteriology·James M Dubbs, Skorn Mongkolsuk
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Mar 7, 2006·Applied and Environmental Microbiology·Obidimma C EzezikaEllen L Neidle
Aug 23, 2017·Scientific Reports·Andrea CillingováJozef Nosek
Mar 17, 2021·Chemical Reviews·Jigneshkumar Dahyabhai PrajapatiMathias Winterhalter
May 12, 2009·Journal of Bacteriology·Hung-Kuang ChangGerben J Zylstra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.