The beta-1,3-glucanosyltransferase gas4p is essential for ascospore wall maturation and spore viability in Schizosaccharomyces pombe

Molecular Microbiology
María de Medina-RedondoCarlos R Vázquez de Aldana

Abstract

Meiosis is the developmental programme by which sexually reproducing diploid organisms generate haploid gametes. In yeast, meiosis is followed by spore morphogenesis. The formation of the Schizosaccharomyces pombe ascospore wall requires the co-ordinated activity of enzymes involved in the biosynthesis and modification of its components, such as glucans. During sporogenesis, the beta-1,3-glucan synthase bgs2p synthesizes linear beta-1,3-glucans, which remain unorganized and alkali-soluble until covalent linkages are set up between beta-1,3-glucans and other cell wall components. Several proteins belonging to the glycoside hydrolase family 72 (GH72) with beta-1,3-glucanosyltransferase activity have been described in other organisms, such as the Saccharomyces cerevisiae Gas1p or the Aspergillus fumigatus Gel1p. Here we describe the characterization of gas4(+), a new gene that encodes a protein of the GH72 family. Deletion of this gene does not lead to any apparent defect during vegetative growth, but homozygous gas4Delta diploids show a sporulation defect. Although meiosis occurs normally, ascospores are unable to mature or to germinate. The expression of gas4(+) is strongly induced during sporulation and a yellow fluorescent pro...Continue Reading

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Citations

Apr 27, 2012·Medical Microbiology and Immunology·Leah R VillegasAndrew H Limper
Dec 23, 2008·The Journal of Biological Chemistry·Ramon Hurtado-GuerreroDaan M F van Aalten
Jun 15, 2010·Eukaryotic Cell·Amandine GasteboisIsabelle Mouyna
Sep 21, 2010·Eukaryotic Cell·Encarnación Dueñas-SanteroCarlos Vázquez de Aldana
Jan 1, 2013·Fungal Genetics and Biology : FG & B·Masayuki KameiMakoto Fujimura
Dec 28, 2010·Fungal Genetics and Biology : FG & B·Laura Alcazar-FuoliJean-Paul Latgé
Aug 21, 2015·Current Opinion in Microbiology·Valeria Davì, Nicolas Minc
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Feb 26, 2010·FEMS Microbiology Letters·Mohammad Reza SharifmoghadamMaría-Henar Valdivieso
Jul 25, 2017·Cold Spring Harbor Protocols·Pilar Pérez, Juan C Ribas
Mar 20, 2016·Journal of Phycology·Matthias T BuhmannPeter G Kroth
Dec 2, 2020·International Journal of Molecular Sciences·Renata TeparićVladimir Mrša

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