The beta-3 adrenergic agonist (CL-316,243) restores the expression of down-regulated fatty acid oxidation genes in type 2 diabetic mice

Nutrition & Metabolism
Amit KumarEmily J Gallagher

Abstract

The hallmark of Type 2 diabetes (T2D) is hyperglycemia, although there are multiple other metabolic abnormalities that occur with T2D, including insulin resistance and dyslipidemia. To advance T2D prevention and develop targeted therapies for its treatment, a greater understanding of the alterations in metabolic tissues associated with T2D is necessary. The aim of this study was to use microarray analysis of gene expression in metabolic tissues from a mouse model of pre-diabetes and T2D to further understand the metabolic abnormalities that may contribute to T2D. We also aimed to uncover the novel genes and pathways regulated by the insulin sensitizing agent (CL-316,243) to identify key pathways and target genes in metabolic tissues that can reverse the diabetic phenotype. Male MKR mice on an FVB/n background and age matched wild-type (WT) FVB/n mice were used in all experiments. Skeletal muscle, liver and fat were isolated from prediabetic (3 week old) and diabetic (8 week old) MKR mice. Male MKR mice were treated with CL-316,243. Skeletal muscle, liver and fat were isolated after the treatment period. RNA was isolated from the metabolic tissues and subjected to microarray and KEGG database analysis. Significant decreases in t...Continue Reading

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Citations

May 21, 2016·Journal of Diabetes and Its Complications·Emily J GallagherJoseph Shiloach
Sep 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xuan YangXiaoyun He
Jul 11, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Narumi KuboKazuki Fukuchi
May 23, 2021·Alzheimer's Research & Therapy·Marine TournissacFrédéric Calon

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Methods Mentioned

BETA
transgenic
Chip

Software Mentioned

MATLAB
Gene Chip Operating
Partek
SigmaStat
Ingenuity Pathway Analysis ( IPA )

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