The beta-galactoside binding immunomodulatory lectin galectin-3 reverses the desensitized state induced in neutrophils by the chemotactic peptide f-Met-Leu-Phe: role of reactive oxygen species generated by the NADPH-oxidase and inactivation of the agonist

Glycobiology
Huamei ForsmanC Dahlgren

Abstract

Neutrophils interacting with a chemoattractant gradually become nonresponsive to further stimulation by the same agonist, a process known as desensitization. Receptor desensitization is a highly regulated process that involves different mechanisms depending on which receptor-ligand pair that is studied. Galectin-3, a member of a large family of beta-galactoside-binding lectins, has been suggested to be a regulator of the inflammatory process, augmenting or directly triggering the neutrophil functional repertoire. We show here that the desensitized state of neutrophils interacting with the chemotactic peptide fMLF is broken by galectin-3 and that this is achieved through an oxygen radical-mediated inactivation of the chemoattractant. The effect was inhibited by the competitor lactose and required the affinity of galectin-3 for N-acetyllactosamine, a saccharide typically found on cell surface glycoproteins. The latter was shown using a galectin-3 mutant that lacked N-acetyllactosamine binding activity, and this protein was not active. The mechanism behind the inactivation of the chemoattractant was found to depend on the ability of galectin-3 to induce a neutrophil generation/secretion of reactive oxygen species which in combined...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E SchiffmannS M Wahl
May 16, 1990·Biochemical and Biophysical Research Communications·F BoulayP Vignais
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I GoldsteinG Weissmann
Jan 1, 1994·Annual Review of Immunology·P M Murphy
May 30, 1994·Biochemical and Biophysical Research Communications·M DurstinP M Murphy
May 28, 1998·The Journal of Biological Chemistry·J SeetharamanJ M Rini
Feb 26, 1999·The Journal of Biological Chemistry·H AliR Snyderman
Jan 5, 2000·Journal of Immunological Methods·C Dahlgren, A Karlsson
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenny AlmkvistAnna Karlsson
Feb 6, 2004·Glycoconjugate Journal·Jenny Almkvist, Anna Karlsson
Oct 27, 2004·Advances in Protein Chemistry·Aaron F Miller, Joseph J Falke
Mar 19, 2005·International Archives of Allergy and Immunology·Fu-Tong Liu
Dec 21, 2005·Journal of Leukocyte Biology·Huamei FuClaes Dahlgren
Feb 16, 2006·Biochimica Et Biophysica Acta·Jerka DumicMirna Flögel
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fredrik B ThorénKristoffer Hellstrand
Sep 14, 2006·PLoS Medicine·Malin HultqvistRikard Holmdahl
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huan-Yuan ChenFu-Tong Liu
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joseph D HernandezLinda G Baum
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fredrik B ThorénKristoffer Hellstrand
Aug 7, 2007·Antioxidants & Redox Signaling·Kyra A GeldermanRikard Holmdahl
Oct 20, 2007·Journal of Neuroimmunology·Natalia MossbergCharlotta Movitz
Nov 10, 2007·Drug News & Perspectives·Fu-Tong Liu, Daniel K Hsu
Jan 19, 2008·The American Journal of Pathology·Sarah L FarnworthTariq Sethi

❮ Previous
Next ❯

Citations

Mar 12, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ahdeah Pajoohesh-Ganji, Kimberly R Byrnes
Sep 3, 2010·The Journal of Biological Chemistry·Emma SalomonssonHakon Leffler
Sep 3, 2002·American Journal of Respiratory and Critical Care Medicine·Victoria del PozoCarlos Lahoz
Sep 28, 2010·Free Radical Biology & Medicine·Johan BylundAnna Karlsson
Sep 9, 2011·Immunobiology·Billur BaserasGertrud Maria Hänsch
Oct 26, 2005·FEBS Letters·Klaas P J M van GisbergenYvette van Kooyk
Aug 21, 2007·Neurobiology of Disease·Caroline L BellacStephen L Leib
Mar 16, 2017·Frontiers in Pediatrics·Shelley Melissa LawrenceVictor Nizet
Aug 24, 2019·Frontiers in Immunology·Brian S RobinsonSean R Stowell
Oct 21, 2019·Molecular Immunology·Lílian C RodriguesMarcelo Dias-Baruffi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.