Aug 1, 1976

The beta-glucosidase in the gut contents of the snail Achatina achatina

The Biochemical Journal
G M Umezurike


1. The enzyme beta-glucosidase (beta-D-glucoside glucohydrolase, EC from the gut contents of active Achatina achatina exists in two molecular forms, beta-glucosidase C (mol.wt. about 82000) and D (mol.wt. about 41000). 2. Only the lower-molecular-weight species was found in the gut contents of aestivating snails or in extracts from their digestive glands and washed gut walls. 3. On re-activation of some aestivating snails, betion of ATP and Mg2+ to the isolated gut contents or to extracts from washed gut walls led to the formation of higher-molecular-weight forms of the enzyme, beta-glucosidase A (mol.wt. about 329000) and beta-glucosidase B (mol.wt. about 165000). 5. All these forms of the enzyme have similar pH optimum (pH 5.0-5.6). 6. The Michaelis constants (Km) and heat stability of the enzyme increased with increasing molecular complexity.

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Mentioned in this Paper

Helix (Snails)
Gastrointestinal System
SNAI1 gene

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