The Beta-subunit of the rabbit H,K-ATPase:a glycoprotein with all terminal lactosamine units capped with alpha-linked galactose residues

Biochemistry
K TyagarajanJ G Forte

Abstract

The beta-subunit of the gastric H,K-ATPase is the most abundant glycoprotein in the tubulovesicular compartment of the acid-secreting parietal cells. The oligosaccharides of the beta-subunit have been shown to contain fucose, N-acetylglucosamine, mannose, galactose, and N-acetylgalactosamine. Previous studies have shown that the rabbit beta-subunit is devoid of N-acetylneuraminic acid. Here we report the structural features of the N-linked oligosaccharides of the beta-subunit from rabbit H,K-ATPase. We used glycosidase digestions and analysis by high-pH anion-exchange chromatography with pulsed amperometric detection and matrix-assisted laser desorption/ionization mass spectrometry to analyze the peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase (PNGase F)- and endo-beta-N-acetylglucosaminidase H (Endo H)-released oligosaccharides. The studies showed that the oligosaccharides of the beta-subunit are a mixture of both oligomannosidic and lactosamine-type structures. The high-mannose structures were identified as Man5Man8GlcNAc2 species. A striking finding was that all the branches of the lactosamine-type structures were terminated with Galalpha-->Galbeta-->GlcNAc extensions. All of the lactosamine-type structures were ...Continue Reading

References

Apr 15, 1989·European Journal of Biochemistry·U V SanterM C Glick

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Citations

Apr 19, 2007·Journal of Molecular Histology·Giuseppa Esterina LiquoriDomenico Ferri
Nov 21, 2007·Infection and Immunity·Sharon M TennantIan R van Driel
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maria MastrodonatoDomenico Ferri
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Sep 1, 2008·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Lixin Zhu
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Jun 11, 2019·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takuto FujiiHideki Sakai

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