The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes

Plant Molecular Biology
P J HusseyC D Silflow

Abstract

Maize beta-tubulins are encoded by a large multigene family with at least nine members, as determined by Southern blot analysis. Two expressed genes, represented by the beta 1 genomic clone and the beta 2 cDNA clone, were examined in this study. The two genes encode beta-tubulins which show 94% sequence identity at the amino acid level. Maize beta 1 transcript levels were highest in seedling root tip and tissue culture cells, which are both rapidly dividing tissues. No transcripts were detected in non-dividing leaf tissue. In contrast, beta 2 transcripts were present at relatively high levels in tissue culture cells and at lower levels in seedling root tip and leaf tissue. The electrophoretic mobility of the beta 2 polypeptide was examined in relation to the constellation of beta-tubulin polypeptides on two-dimensional gel western blots of a maize pollen total protein extract. No evidence for post-translational modification of the beta-tubulin polypeptides was found in pollen.

References

Dec 20, 1979·Nucleic Acids Research·J P GergenP C Wensink
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R P RicciardiB E Roberts
Apr 1, 1989·Genes & Development·J C LarkinC D Silflow
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S R LudwigD P Snustad
Jun 25, 1986·Nucleic Acids Research·M D McMullenI Rubenstein
Jan 1, 1985·Annual Review of Biochemistry·D W Cleveland, K F Sullivan
Jan 1, 1985·Cell Biology International Reports·K Mizuno
Dec 1, 1984·Molecular and Cellular Biology·J YoungblomC D Silflow
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Feb 1, 1982·Molecular and Cellular Biology·H Okayama, P Berg
Jul 1, 1984·The Journal of Cell Biology·E C Raff
Mar 1, 1982·Gene·N Hu, J Messing

❮ Previous
Next ❯

Citations

Dec 1, 1994·Plant Molecular Biology·M S KangM J Cho
Feb 1, 1995·Plant Molecular Biology·H L BrierleyS R Long
Oct 1, 1993·Plant Molecular Biology·M G LiD P Snustad
Dec 1, 1996·Plant Molecular Biology·T D VassilevskaiaC Rodrigues-Pousada
Jul 1, 1993·Plant Molecular Biology·T D VassilevskaiaC Rodrigues-Pousada
Jan 1, 1991·Pharmacology & Therapeutics·L C Morejohn, D E Fosket
Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·R G Anthony, P J Hussey
May 1, 1993·Molecular Biology of the Cell·R F Ludueña
Jun 23, 1998·The Plant Cell·B C GibbonC J Staiger
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Peter W. Barlow, Frantisek Baluska
Jan 5, 2002·Bioscience, Biotechnology, and Biochemistry·B G KimY Magae
Dec 3, 2014·Biochemical and Biophysical Research Communications·Mitra Shojania Feizabadi, Yonggun Jun
Mar 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N MenduC D Silflow
Jan 9, 2004·Applied and Environmental Microbiology·Jaimie M Warren, Sarah F Covert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.