The beta-tubulin gene family of Arabidopsis thaliana: preferential accumulation of the beta 1 transcript in roots

Gene
D G OppenheimerD P Snustad

Abstract

The genome of Arabidopsis thaliana (L.) Heynh. was shown to contain a beta-tubulin gene family consisting of at least seven distinct genes and/or pseudogenes. Genomic clones of five different beta-tubulin genes and/or pseudogenes have been isolated and partially characterized. The complete nucleotide sequence of one A. thaliana beta-tubulin gene, designated beta 1, has been determined. A comparison of the predicted amino acid sequence of the A. thaliana beta 1-tubulin with the predicted sequences of beta-tubulins of animals and protists indicated that this plant beta-tubulin shows a high degree of homology with other beta-tubulins. However, the beta 1-tubulin contains a novel single amino acid insertion at position 41. The A. thaliana beta 1-tubulin gene is transcribed, as shown by RNA blot hybridization and S1 nuclease analyses. A 3'-noncoding gene-specific probe was used to examine the expression of the beta 1-tubulin gene in leaves, roots, and flowers by blot hybridization analyses of total RNA isolated from these tissues. The results showed that the transcript of the beta 1 gene accumulates predominantly in roots, with low levels of transcript in flowers, and barely detectable levels of transcript in leaves. A second genomi...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R WeinstockR Axel
Jan 1, 1975·Annual Review of Genetics·G P Rédei
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C Chang, E M Meyerowitz
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K F Sullivan, D W Cleveland
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S R LudwigD P Snustad
Jan 20, 1986·Journal of Molecular Biology·R E Pruitt, E M Meyerowitz
Jun 25, 1986·Nucleic Acids Research·M D McMullenI Rubenstein
Jan 1, 1985·Annual Review of Biochemistry·D W Cleveland, K F Sullivan
Oct 25, 1985·Nucleic Acids Research·K C Reed, D A Mann
Jan 1, 1973·Annual Review of Biochemistry·J B Olmsted, G G Borisy
Jan 1, 1974·Triangle; the Sandoz Journal of Medical Science·O Behnke
Jun 4, 1974·Biochemistry·V GlisinC Byus
Dec 1, 1984·Molecular and Cellular Biology·J YoungblomC D Silflow
Jul 1, 1984·The Journal of Cell Biology·L C Morejohn, D E Fosket
Apr 1, 1980·Analytical Biochemistry·J LangridgeP L Bergquist
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Jan 1, 1983·Methods in Enzymology·J E Walker, N J Gay
Jul 1, 1984·The Journal of Cell Biology·E C Raff
Jul 1, 1984·The Journal of Cell Biology·S C TiwariB E Gunning
Mar 1, 1982·Gene·N Hu, J Messing
Jan 22, 1982·Nucleic Acids Research·S M Mount
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L C MorejohnD E Fosket
Sep 20, 1985·Science·E M Meyerowitz, R E Pruitt

❮ Previous
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Citations

Jan 1, 1990·Plant Molecular Biology·L MontoliuP Puigdomènech
Dec 1, 1994·Plant Molecular Biology·M S KangM J Cho
Feb 1, 1995·Plant Molecular Biology·H L BrierleyS R Long
Apr 1, 1991·Plant Molecular Biology·T D SutliffR L Rodriguez
Mar 1, 1993·Plant Molecular Biology·J L CarpenterC D Silflow
Oct 1, 1993·Plant Molecular Biology·M G LiD P Snustad
Aug 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J M McGrathE Pichersky
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·J GloverE Dennis
May 1, 1992·The Plant Cell·J L CarpenterC D Silflow
Apr 23, 1991·European Journal of Biochemistry·H SoaresC Rodrigues-Pousada
May 6, 2003·Annual Review of Phytopathology·Howard J AtkinsonMichael J McPherson
Jan 5, 2002·Bioscience, Biotechnology, and Biochemistry·B G KimY Magae
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Janice de Almeida EnglerGodelieve Gheysen
Mar 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N MenduC D Silflow
Jan 10, 2002·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·S R MitznerR Schmidt

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