The Bicoid class homeodomain factors ceh-36/OTX and unc-30/PITX cooperate in C. elegans embryonic progenitor cells to regulate robust development

PLoS Genetics
Travis WaltonJohn I Murray

Abstract

While many transcriptional regulators of pluripotent and terminally differentiated states have been identified, regulation of intermediate progenitor states is less well understood. Previous high throughput cellular resolution expression studies identified dozens of transcription factors with lineage-specific expression patterns in C. elegans embryos that could regulate progenitor identity. In this study we identified a broad embryonic role for the C. elegans OTX transcription factor ceh-36, which was previously shown to be required for the terminal specification of four neurons. ceh-36 is expressed in progenitors of over 30% of embryonic cells, yet is not required for embryonic viability. Quantitative phenotyping by computational analysis of time-lapse movies of ceh-36 mutant embryos identified cell cycle or cell migration defects in over 100 of these cells, but most defects were low-penetrance, suggesting redundancy. Expression of ceh-36 partially overlaps with that of the PITX transcription factor unc-30. unc-30 single mutants are viable but loss of both ceh-36 and unc-30 causes 100% lethality, and double mutants have significantly higher frequencies of cellular developmental defects in the cells where their expression norma...Continue Reading

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Citations

Sep 19, 2015·Molecular Reproduction and Development·Morris F Maduro
Mar 2, 2016·BMC Genomics·Joshua BurdickJohn Isaac Murray
May 27, 2015·Lab on a Chip·Jinzhou YuanHaim H Bau
Jan 26, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·John Isaac Murray
May 3, 2016·Wiley Interdisciplinary Reviews. Developmental Biology·Oliver Hobert
Feb 27, 2016·Genesis : the Journal of Genetics and Development·Amanda L Zacharias, John Isaac Murray
Sep 14, 2016·Developmental Cell·Inna V NechipurenkoPiali Sengupta
Jul 17, 2020·Heliyon·Jacob R Manjarrez, Roger Mailler
Aug 24, 2021·Development·Karolina MizerackaMaxwell G Heiman

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Methods Mentioned

BETA
confocal microscopy
transgenic
1-hybrid
PCR
dissection

Software Mentioned

AceTree
LASAF
StarryNite

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