The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells

BMC Cancer
Jeong Yong JeonMijin Yun

Abstract

Recent reports using metabolism regulating drugs showed that nutrient deprivation was an efficient tool to suppress cancer progression. In addition, autophagy control is emerging to prevent cancer cell survival. Autophagy breaks down the unnecessary cytoplasmic components into anabolic units and energy sources, which are the most important sources for making the ATP that maintains homeostasis in cancer cell growth and survival. Therefore, the glucose analog 2-deoxyglucose (2DG) has been used as an anticancer reagent due to its inhibition of glycolysis. Prostate cancer cells (PC3) were treated with 2DG for 6 h or 48 h to analyze the changing of cell cycle and autophagic flux. Rapamycin and LC3B overexpressing vectors were administered to PC3 cells for autophagy induction and chloroquine and shBeclin1 plasmid were used to inhibit autophagy in PC3 cells to analyze PC3 cells growth and survival. The samples for western blotting were prepared in each culture condition to confirm the expression level of autophagy related and regulating proteins. We demonstrated that 2DG inhibits PC3 cells growth and had discriminating effects on autophagy regulation based on the different time period of 2DG treatment to control cell survival. Short-t...Continue Reading

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Citations

Jun 21, 2017·International Journal of Molecular Sciences·Ying-Ying LiNiramol Savaraj
Mar 6, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peng ZhangYifei Xing
Sep 19, 2018·ChemMedChem·Serena FortunatoFilippo Minutolo
Apr 17, 2019·Journal of Experimental & Clinical Cancer Research : CR·Luigi MeleFederica Papaccio
Dec 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yangke WanyanYuqing Chen

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Methods Mentioned

BETA
electrophoresis
confocal microscopy
FACS
genetic modification
chemical

Software Mentioned

GraphPad Prism

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