The Binding of CD93 to Multimerin-2 Promotes Choroidal Neovascularization

Investigative Ophthalmology & Visual Science
Gian Marco TosiMaurizio Orlandini

Abstract

The purpose of this study was to investigate the involvement of CD93 and Multimerin-2 in three choroidal neovascularization (CNV) models and to evaluate their contribution in the neovascular progression of age-related macular degeneration (AMD). Choroidal neovascular membranes collected during surgery from AMD patients were analyzed by microscopy methods. Laser-induced CNV mouse models and choroid sprouting assays (CSAs) were carried out using the CD93 knockout mouse model. An original ex vivo CSA of vascular angiogenesis, employing choroid tissues isolated from human donors, was developed. In contrast to healthy choroid endothelium, hyperproliferative choroidal endothelial cells (ECs) of AMD patients expressed high levels of CD93, and Multimerin-2 was abundantly deposited along the choroidal neovasculature. CD93 knockout mice showed a significant reduced neovascularization after laser photocoagulation, and their choroidal ECs displayed a decreased ability to produce sprouts in ex vivo angiogenesis assays. Moreover, the presence of an antibody able to hamper the CD93/Multimerin-2 interaction reduced vascular sprouting in the human CSA. Our results demonstrate that CD93 and its interaction with Multimerin-2 play an important rol...Continue Reading

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Citations

Jun 2, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·Stefano BarberaMaurizio Orlandini

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy

Software Mentioned

GraphPad
Prism
ImageJ
NIS
Elements

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