PMID: 40792Sep 1, 1979

The binding of chloride ions to ligated and unligated human hemoglobin and its influence on the Bohr effect

European Journal of Biochemistry
G G Van BeekS H De Bruin

Abstract

The contribution of the interaction of chloride ions with deoxy and oxyhemoglobin to the Bohr effect can be described by a simple binding model. Applying this model to experiment data reveals that at physiological pH and ionic strength about half of the release of Bohr protons is due to a difference in chloride ion binding to deoxy- and oxyhemoglobin. The chloride-independent part of the Bohr effect corresponds with the shift in pK which His-146 beta shows upon oxygenation. The proton absorptioon by hemoglobin observed upon oxygenation below pH 6 is apparently due to a chloride-ion-induced proton uptake, which is larger for oxyhemoglobin than for deoxyhemoglobin. The analysis of the experimental data indicates the existence of only two oxygen-linked chloride ion binding sites in both deoxy and oxyhemoglobin. In deoxyhemoglobin the binding sites most likely consist of Val-1 alpha of one chain and Arg-141 alpha of the partner chain. The sites in oxyhemoglobin consist of groups with a pK value in the neutral pH range; they do not contain lysyl or arginyl residues.

References

Dec 4, 2012·Antioxidants & Redox Signaling·Celia BonaventuraAlvin L Crumbliss
Apr 15, 1980·Journal of Molecular Biology·M F PerutzH S Rollema
Jun 19, 2002·Blood·Kevin E SandersStephen G Sligar

Citations

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R N Haire, B E Hedlund
Oct 5, 1977·Journal of Molecular Biology·A ArnoneR Benesch
May 1, 1969·Biochemistry·J M HalloinE R Fowlks
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J V KilmartinC Ho
Nov 1, 1973·Biochemical and Biophysical Research Communications·S H De BruinG A van Os
May 7, 1974·Biochemical and Biophysical Research Communications·S H De BruinG A van Os
Aug 14, 1971·Journal of Molecular Biology·G L Kellett
Jan 1, 1964·Advances in Protein Chemistry·J Wyman

Related Concepts

Chloride Ion Level
Protons
Eryhem
Oxidation-Reduction
Hydrogen-Ion Concentration

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