PMID: 6973966Dec 1, 1980Paper

The binding of lectins to sheep tissues and circulating cells: peanut agglutinin, a marker for presumptive T-lymphocytes

The Australian Journal of Experimental Biology and Medical Science
K J Fahey

Abstract

A study was undertaken into the binding of 7 fluorescein (FITC)-labelled, lectins to sheep tissues and cells. Wheat germ agglutinin (WGA), conconavalin A (con A) and Ricinus communis agglutinin (RCA) bound strongly to all sheep tissues and circulating cells, while Dolichos biflorus (DBA) and Ulex europaeus (UEA) did not bind at all. Peanut agglutinin (PNA) and soybean agglutinin (SBA) bound to thymus and lymph node cell suspensions and to a proportion of circulating cells, but only weakly to the other sheep tissues tested. PNA and SBA bound to polymorphs and monocytes, making it necessary to treat whole blood with carbonyl-iron and magnet to remove the phagocytic cells before purfication of the peripheral blood leucocytes (PBL). PNA bound to 40 to 50% of carbonyl-iron treated PBL and to 65 to 70% of the cells in efferent popliteal lymph. Using a rhodamine-labelled anti-sheep lg reagent greater than 98% of PNA + cells in lymph and carbonyl-iron treated PBL were sIg-. Furthermore, using an FITC-labelled F(ab')2 anti-sheep Ig reagent, the sum of the 0/0 PNA+cells and sIg+ cells in individually labelled preparations was equal to the percentage of cells labelled when both reagents were added simultaneously. Cells binding PNA were fo...Continue Reading

Citations

Jun 1, 1988·International Journal for Parasitology·H J DawkinsJ K Dineen
Jan 1, 1982·Veterinary Immunology and Immunopathology·R M Binns
Oct 1, 1989·Veterinary Immunology and Immunopathology·S Djilali, A L Parodi
Apr 1, 1994·Veterinary Immunology and Immunopathology·R WilkinsonM Barton
Jul 1, 1982·Journal of Comparative Pathology·M R BatungbacalC Burrells
Nov 1, 1987·Journal of Comparative Pathology·C McColganH R Miller
Jun 1, 1986·Immunological Reviews·M Miyasaka, Z Trnka
Nov 17, 1986·European Journal of Biochemistry·P MoullierJ Hoebeke
Jul 20, 2002·The Journal of Veterinary Medical Science·Kwang-Il JeongChanhee Chae

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