PMID: 8609168Apr 1, 1996Paper

The binding of plakoglobin to desmosomal cadherins: patterns of binding sites and topogenic potential

The Journal of Cell Biology
N A ChitaevS M Troyanovsky

Abstract

Plakoglobin is the only protein that occurs in the cytoplasmic plaques of all known adhering junctions and has been shown to be crucially involved in the formation and maintenance of desmosomes anchoring intermediate-sized filaments (IFs) by its interaction with the desmosomal cadherins, desmoglein (Dsg), and desmocollin (Dsc). This topogenic importance of plakoglobin is now directly shown in living cells as well as in binding assays in vitro. We show that, in transfected human A-431 carcinoma cells, a chimeric protein combining the vesicle-forming transmembrane glycoprotein synaptophysin, with the complete human plakoglobin sequence, is sorted to small vesicles many of which associate with desmosomal plaques and their attached IFs. Immunoprecipitation experiments have further revealed that the chimeric plakoglobin-containing transmembrane molecules of these vesicles are tightly bound to Dsg and Dsc but not to endogenous plakoglobin, thus demonstrating that the binding of plakoglobin to desmosomal cadherins does not require its soluble state and is strong enough to attach large structures such as vesicles to desmosomes. To identify the binding domains and the mechanisms involved in the interaction of plakoglobin with desmosomal...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·W W FrankeR E Leube
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J KochW W Franke
Jan 1, 1990·Annual Review of Biochemistry·M Takeichi
Jan 1, 1990·Annual Review of Cell Biology·M A SchwarzW W Franke
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M OzawaR Kemler
Oct 1, 1985·The Journal of Cell Biology·P CowinW W Franke
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·W W FrankeC Stähli
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeP Cowin
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·O RoseW W Franke
Aug 25, 1995·The Journal of Biological Chemistry·P A SaccoK R Johnson
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·T S JouJ A Marrs
Dec 1, 1994·The Journal of Cell Biology·R E LeubeB Wiedenmann
Dec 1, 1994·The Journal of Cell Biology·J HülskenJ Behrens
Aug 1, 1994·Cell Adhesion and Communication·J Stappert, R Kemler
Oct 1, 1994·Current Opinion in Cell Biology·P J Koch, W W Franke
Mar 1, 1995·The Journal of Cell Biology·S ShibamotoM Takeichi
Dec 1, 1994·Differentiation; Research in Biological Diversity·H W HeidW W Franke
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M TroyanovskyW W Franke
Feb 1, 1993·Current Opinion in Cell Biology·D R Garrod
May 1, 1993·The Journal of Cell Biology·R S BuxtonJ R Stanley

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Citations

Oct 18, 2001·Clinics in Dermatology·E CozzaniA Parodi
Sep 17, 2002·Current Opinion in Cell Biology·David R GarrodZhuxiang Nie
Oct 1, 1996·Current Opinion in Cell Biology·D GarrodA North
Feb 21, 2002·The Biochemical Journal·Shabih-e-Hassnain SyedAnthony I Magee
Oct 8, 1999·Molecular Biology of the Cell·M W KlymkowskyY E Vourgourakis
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Emmanuella DelvaAndrew P Kowalczyk
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Werner W Franke
May 17, 2008·Pharmacological Reviews·Dolores D MrukC Yan Cheng
Dec 16, 2003·Molecular and Cellular Biology·Xing ChengPeter J Koch
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Margaret J Wheelock, Keith R Johnson
Dec 29, 2000·Molecular Membrane Biology·N SerpenteA I Magee
Feb 14, 1998·The Journal of Cell Biology·P WhiteJ P Vincent
Oct 14, 2004·The Journal of Cell Biology·Katja S GrossmannWalter Birchmeier
Aug 12, 1998·The Journal of Cell Biology·N A Chitaev, S M Troyanovsky
Jun 12, 1998·The Journal of Cell Biology·E A Smith, E Fuchs
Feb 24, 1997·The Journal of Cell Biology·J E LewisM J Wheelock
Apr 4, 2000·The Journal of Cell Biology·M HatzfeldU Vinzens
Jan 28, 2014·Cell Communication & Adhesion·Maranke I KosterPeter J Koch
May 15, 2007·The Journal of Investigative Dermatology·Christian J SchmittWiebke K Peitsch
Jun 10, 2004·The Journal of Dermatology·Xing Cheng, Peter J Koch
Jan 28, 2004·Journal of Cutaneous Pathology·Hjalmar KurzenWolfgang Hartschuh
Sep 15, 2007·Biochimica Et Biophysica Acta·David Garrod, Martyn Chidgey
Dec 23, 2008·Developmental Biology·Eva D MartinMaura Grealy
May 1, 1997·Developmental Biology·J M MerriamM W Klymkowsky
Feb 24, 2015·The Journal of Clinical Investigation·Zhiwei ZhangJu Chen
Oct 3, 2002·Journal of Cutaneous Pathology·Lorenzo Lo MuzioGaetano De Rosa
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·K J Green, C A Gaudry
Apr 23, 2004·Molecular Pharmacology·Xing-Jie LiangMichael M Gottesman

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