The biochemical and mass spectrometric profiling of the dystrophin complexome from skeletal muscle

Computational and Structural Biotechnology Journal
Sandra Murphy, Kay Ohlendieck

Abstract

The development of advanced mass spectrometric methodology has decisively enhanced the analytical capabilities for studies into the composition and dynamics of multi-subunit protein complexes and their associated components. Large-scale complexome profiling is an approach that combines the systematic isolation and enrichment of protein assemblies with sophisticated mass spectrometry-based identification methods. In skeletal muscles, the membrane cytoskeletal protein dystrophin of 427 kDa forms tight interactions with a variety of sarcolemmal, cytosolic and extracellular proteins, which in turn associate with key components of the extracellular matrix and the intracellular cytoskeleton. A major function of this enormous assembly of proteins, including dystroglycans, sarcoglycans, syntrophins, dystrobrevins, sarcospan, laminin and cortical actin, is postulated to stabilize muscle fibres during the physical tensions of continuous excitation-contraction-relaxation cycles. This article reviews the evidence from recent proteomic studies that have focused on the characterization of the dystrophin-glycoprotein complex and its central role in the establishment of the cytoskeleton-sarcolemma-matrisome axis. Proteomic findings suggest a c...Continue Reading

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Citations

Sep 8, 2016·Expert Review of Proteomics·Elena L RudashevskayaStavroula Markoutsa
Jan 18, 2017·Der Pathologe·K Ohlendieck, D Swandulla
Feb 13, 2018·Tissue Engineering. Part a·Michael J McClureBarbara D Boyan
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Jan 27, 2019·Expert Review of Proteomics·Sandra MurphyKay Ohlendieck
Mar 20, 2019·Journal of Muscle Research and Cell Motility·Sandra MurphyKay Ohlendieck
Jul 31, 2019·Expert Review of Molecular Diagnostics·Paul DowlingKay Ohlendieck
Aug 7, 2017·Expert Review of Proteomics·Daniele CapitanioCecilia Gelfi
Feb 19, 2020·Expert Review of Proteomics·Paul DowlingKay Ohlendieck
Jan 18, 2018·Expert Review of Proteomics·Sandra MurphyKay Ohlendieck
Oct 8, 2020·GeroScience·Jan Frohlich, Manlio Vinciguerra
Sep 15, 2020·IScience·Hathaichanok TamiyakulThomas Fröhlich
Sep 24, 2021·Pflügers Archiv : European journal of physiology·Kay Ohlendieck, Dieter Swandulla
Sep 15, 2021·Endocrine Reviews·Buel D Rodgers, Christopher W Ward

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Methods Mentioned

BETA
proteomic
biopsies
electrophoresis
ion exchange chromatography
Co-immunoprecipitation
immunoprecipitation
transgenic
affinity purification

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