PMID: 3730052Jul 1, 1986

The biochemistry of epsilon-amino groups of lysine residues from apolipoprotein B of human low density lipoprotein

Atherosclerosis
E Cocuzzi, W C Breckenridge

Abstract

The availability of epsilon-lysine residues of apolipoprotein B in LDL for chemical or enzymic modification was investigated. Amino acid analyses of detergent-solubilized apolipoprotein B, following cyanoethylation with acrylonitrile, revealed that 10% of the lysine in apolipoprotein B were unreactive. The unreactive residues were associated with the most hydrophobic subfraction of apolipoprotein B. Since apolipoprotein B has a high molecular weight a study was undertaken to determine whether lysine residues were crosslinked to glutamic acid via epsilon-(gamma-glutamyl)lysine as demonstrated for fibrin. Apolipoprotein B was digested exhaustively with proteases. The content of epsilon-(gamma-glutamyl) lysine was determined by chromatography and isotope dilution. In contrast to earlier reports for serum LDL the data showed that less than 0.01 moles of lysine/mole of LDL apolipoprotein B were present as epsilon-(gamma-glutamyl)lysine in plasma LDL. It was determined also that the crosslinks were not found in apolipoprotein B during clotting since LDL was not a substrate for clotting factor XIII which forms the bond in fibrin. Furthermore, the lipoprotein contained no inherent transglutaminase activity. It is concluded that the lys...Continue Reading

References

Nov 30, 2010·Journal of Obesity·Stephen H Boutcher
Feb 1, 1987·American Heart Journal·W C Breckenridge

Citations

Dec 8, 1972·Annals of the New York Academy of Sciences·K LakiJ Farrell
Dec 1, 1972·Analytical Biochemistry·L LorandL Cooperstein
Feb 26, 1968·Biochemical and Biophysical Research Communications·S Matacić, A G Loewy
Sep 26, 1966·Archives of Biochemistry and Biophysics·P A McKeeR L Hill
Jul 1, 1966·Analytical Biochemistry·J V BensonJ A Patterson
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M L FinkJ E Folk

Related Concepts

Fibrin Measurement
Serum LDL
Low Density Lipoprotein Cholesterol Measurement
Nevus
Talpidae
Peptide Hydrolases
Chromatography
N-epsilon-(gamma-L-glutamyl)lysine
Apolipoproteins B
LDL-1

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