The biological basis of degenerative disc disease: proteomic and biomechanical analysis of the canine intervertebral disc

Arthritis Research & Therapy
William Mark ErwinK Arne Mehrkens

Abstract

In the present study, we sought to quantify and contrast the secretome and biomechanical properties of the non-chondrodystrophic (NCD) and chondrodystrophic (CD) canine intervertebral disc (IVD) nucleus pulposus (NP). We used iTRAQ proteomic methods to quantify the secretome of both CD and NCD NP. Differential levels of proteins detected were further verified using immunohistochemistry, Western blotting, and proteoglycan extraction in order to evaluate the integrity of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan. Additionally, we used robotic biomechanical testing to evaluate the biomechanical properties of spinal motion segments from both CD and NCD canines. We detected differential levels of decorin, biglycan, and fibronectin, as well as of other important extracellular matrix (ECM)-related proteins, such as fibromodulin and HAPLN1 in the IVD NP obtained from CD canines compared with NCD canines. The core proteins of the vital SLRPs decorin and biglycan were fragmented in CD NP but were intact in the NP of the NCD animals. CD and NCD vertebral motion segments demonstrated significant differences, with the CD segments having less stiffness and a more varied range of motion. The CD NP recapitulates key ele...Continue Reading

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Citations

Jun 16, 2019·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Kalliopi AlpantakiAlexander Hadjipavlou
Nov 21, 2019·Molecular Medicine Reports·Chang LiuYue Zhou
Sep 16, 2017·Scientific Reports·Joana CaldeiraMário Barbosa
Oct 1, 2017·Experimental and Therapeutic Medicine·Wei WangXianyue Quan
Mar 30, 2017·Neurosurgery·James DowdellSamuel K Cho
Oct 10, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tai-Wei ZhangLi-Bo Jiang
Jan 5, 2021·JOR Spine·Naomi N LeeJames L Cook

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