The biological behavior optimization of human periodontal ligament stem cells via preconditioning by the combined application of fibroblast growth factor-2 and A83-01 in in vitro culture expansion

Journal of Translational Medicine
Chunshu ZhangPishan Yang

Abstract

As the optimal source of seed cells in periodontal tissue engineering, periodontal ligament stem cells (PDLSCs) have always been researched to improve cell expansion due to their limited resource and spontaneous differentiation in vitro cultivation. Fibroblast growth factor-2 (FGF-2) has been proven to stimulate bone marrow mesenchymal stem cells (BMMSCs) proliferation and maintain their pluripotency when being added to the culture medium. As a small molecule inhibitor of transforming growth factor-beta receptors (TGF-βRs), A83-01 can also promote cell proliferation. Therefore, the aim of this study was to verify whether the combined application of FGF-2 and A83-01 could augment cell quantity and quality during in vitro culture. PDLSCs were preconditioned with A83-01, FGF-2, or their combination. A cell counting kit-8 (CCK8) assay, cell apoptosis assay, ALP activity assay, Alizarin Red S staining assay, RT-PCR assay, Western blot assay and ELISA were used to determine the sustained effects of different preconditioning strategies on the proliferation, apoptosis, stemness, osteogenic differentiation and paracrine action of PDLSCs. The combined application of FGF-2 and A83-01 significantly augmented cell expansion, reduced cell ap...Continue Reading

References

Apr 3, 1998·Mineral and Electrolyte Metabolism·A B Roberts
Jan 7, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T KatoK Nakamura
Aug 25, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M L RadomskyJ W Poser
Nov 30, 1999·Journal of Neurosurgery·Y TabataY Ikada
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Feb 7, 2001·The Journal of Clinical Endocrinology and Metabolism·H KawaguchiM Tamura
Aug 10, 2005·Journal of Cellular Biochemistry·Shuan S Huang, Jung S Huang
Mar 15, 2006·BMC Cell Biology·Mandana Mohyeddin BonabBehrouz Nikbin
Apr 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Massimiliano GnecchiVictor J Dzau
Dec 22, 2006·PloS One·Wataru SonoyamaSongtao Shi
Jul 27, 2007·Orthodontics & Craniofacial Research·I C GayM MacDougall
Jul 3, 2008·Stem Cells and Development·Jinping XuSunil Kapila
Dec 31, 2008·Cell Research·Ying E Zhang
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Nov 21, 2009·Cytokine & Growth Factor Reviews·Lindolfo da Silva MeirellesArnold I Caplan
Nov 20, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jie ChenLiangan Chen
Oct 27, 2016·Plastic and Reconstructive Surgery·Ching-Ming ChienYur-Ren Kuo
Aug 23, 2016·Current Stem Cell Reports·Maqsood Ahmed, Charles Ffrench-Constant
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory
Dec 17, 2016·Biochemical and Biophysical Research Communications·Zhijian ZhangXiming Xu
Dec 29, 2016·Tissue Engineering. Part a·Mizuki NagataIkuo Morita

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Protein assay
PCR
ELISA

Software Mentioned

SPSS
Image J

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis