PMID: 3749367Apr 1, 1986Paper

The biological properties of black porgy (Acanthopagrus schlegeli) sperm and its cryopreservation

Proceedings of the National Science Council, Republic of China. Part B, Life Sciences
N H ChaoI C Liao

Abstract

This paper describes the general biology of the testes, milt and spermatozoa of the black porgy, Acanthopagrus schlegeli and reports some preliminary results in which the techniques for cryopreservation of spermatozoa were investigated. During the spawning season from December to February, the gonadosomatic index ranged from 2.0 to 3.5. The milt had an average pH value of 7.4 and osmotic pressure of 385 mOsm/kg. The head of the spermatozoon was apple-shaped and averaged at 1.6 microns in diameter. The best quality of milt was obtained only in the early spawning season. Good motility of spermatozoa could be maintained for up to 10 days in vials hanging in a water bath at 4 degrees C. For cryopreservation, an extender containing 5% glucose mixed with glycerol, serving as the cryoprotective agent (CPA), at a 4:1 ratio was used and the black porgy milt was diluted with the extender at a 1:1 ratio. After an equilibration period no longer than 10 minutes, straws containing this mixture were submerged in isopropanol at -10 degrees C and then frozen at a rate of 2 degrees C/min until the temperature reached -80 degrees C or were held in liquid nitrogen (LN) vapor (-90 to -100 degrees C) for 10 to 20 minutes. A total 720 of 0.5 ml straw...Continue Reading

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