PMID: 7538167Apr 1, 1995Paper

The biological reality of the interlacunar network in the embryonic, cartilaginous, skeleton: a thiazine dye/absolute ethanol/LR White resin protocol for visualizing the network with minimal tissue shrinkage

Journal of Microscopy
D M LawtonJ McClure

Abstract

Third toe phalanges of chicks aged 8-13 days in ovo and 7-day post-natal rat femoral growth plate were examined to determine whether the interlacunar network (IN), a structure with no lipoprotein membrane component or cytoplasmic organelles, is a genuine component of young growth cartilage. In chick phalanges dehydrated by 70% (v/v) ethanol and LR White resin, variable metachromatic staining of the interlacunar network by toluidine blue and red staining by picro-Sirius red indicate the presence of glycosaminoglycans and collagen. The network in phalanges dehydrated by 80% (v/v) ethanol appears little different; however, the network is much less widely detectable in phalanges dehydrated by 90% (v/v) ethanol and, after dehydration by absolute ethanol, is almost completely undetectable. In contrast, when the young cartilage is permeated by a thiazine dye such as toluidine blue, using a solution of dye in the aldehyde fixative, the network is widely detectable, following dehydration by absolute ethanol, both in chick phalanges and in rat growth plate. Comparison of projected areas shows that the extent to which whole chick feet are found to have shrunk, by the time that they are photographed under LR White resin, is determined prin...Continue Reading

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Citations

Apr 5, 2013·PLoS Computational Biology·Brad Busse, Stephen Smith
Feb 20, 2008·Acta Orthopaedica·Anders PalmquistPeter Thomsen
Jun 10, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anders PalmquistPeter Thomsen
Mar 12, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anders PalmquistPeter Thomsen
Jul 16, 2002·Equine Veterinary Journal·Y SunM Hurtig

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