The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni

PLoS Neglected Tropical Diseases
Kathrin K GeyerKarl F Hoffmann

Abstract

The debilitating human disease schistosomiasis is caused by infection with schistosome parasites that maintain a complex lifecycle alternating between definitive (human) and intermediate (snail) hosts. While much is known about how the definitive host responds to schistosome infection, there is comparably less information available describing the snail's response to infection. Here, using information recently revealed by sequencing of the Biomphalaria glabrata intermediate host genome, we provide evidence that the predicted core snail DNA methylation machinery components are associated with both intra-species reproduction processes and inter-species interactions. Firstly, methyl-CpG binding domain protein (Bgmbd2/3) and DNA methyltransferase 1 (Bgdnmt1) genes are transcriptionally enriched in gonadal compared to somatic tissues with 5-azacytidine (5-AzaC) treatment significantly inhibiting oviposition. Secondly, elevated levels of 5-methyl cytosine (5mC), DNA methyltransferase activity and 5mC binding in pigmented hybrid- compared to inbred (NMRI)- B. glabrata populations indicate a role for the snail's DNA methylation machinery in maintaining hybrid vigour or heterosis. Thirdly, locus-specific detection of 5mC by bisulfite (BS...Continue Reading

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Apr 18, 2018·Immunological Reviews·Benjamin GourbalMihai G Netea
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Datasets Mentioned

BETA
EKC32831.1
CK988928
KJ951055
KJ951056

Methods Mentioned

BETA
RNA-Seq
Assay
PCR
electrophoresis
Bisulfite-Seq
glycosylase
RNAseq
WGBS

Software Mentioned

MEGA
R language
BLASTp
MUSCLE ( Sequence Comparison Log
MethPrimer
cNLS mapper
Gblocks
GenomicRanges
MrBayes
GenomicAlignments

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