The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities

Journal of Synchrotron Radiation
S ChenS Vogt

Abstract

Hard X-ray fluorescence microscopy is one of the most sensitive techniques for performing trace elemental analysis of biological samples such as whole cells and tissues. Conventional sample preparation methods usually involve dehydration, which removes cellular water and may consequently cause structural collapse, or invasive processes such as embedding. Radiation-induced artifacts may also become an issue, particularly as the spatial resolution increases beyond the sub-micrometer scale. To allow imaging under hydrated conditions, close to the `natural state', as well as to reduce structural radiation damage, the Bionanoprobe (BNP) has been developed, a hard X-ray fluorescence nanoprobe with cryogenic sample environment and cryo transfer capabilities, dedicated to studying trace elements in frozen-hydrated biological systems. The BNP is installed at an undulator beamline at sector 21 of the Advanced Photon Source. It provides a spatial resolution of 30 nm for two-dimensional fluorescence imaging. In this first demonstration the instrument design and motion control principles are described, the instrument performance is quantified, and the first results obtained with the BNP on frozen-hydrated whole cells are reported.

References

Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D S Gorman, R P Levine
Feb 1, 1995·Quarterly Reviews of Biophysics·J KirzM Howells
May 1, 1995·Acta Crystallographica. Section A, Foundations of Crystallography·D Sayre, H N Chapman
Jan 1, 1993·Journal of Structural Biology·E O'TooleJ R McIntosh
Apr 15, 2003·Nature Materials·Tatjana PauneskuGayle Woloschak
Apr 26, 2003·Journal of Synchrotron Radiation·Tobias Beetz, Chris Jacobsen
Oct 24, 2003·Analytical Chemistry·Benjamin S TwiningSergio A Sañudo-Wilhelmy
Apr 13, 2006·Journal of Synchrotron Radiation·B WinnH Zhang
Sep 29, 2006·Journal of Cellular Biochemistry·Tatjana PauneskuGayle Woloschak
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lydia FinneyDavid Glesne
Jan 17, 2008·Optics Letters·Manuel Guizar-SicairosJames R Fienup
Jun 4, 2008·Physical Review Letters·M D de JongeS Vogt
Jun 17, 2008·Journal of Synchrotron Radiation·B HornbergerS Vogt
Jan 9, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Martina Ralle, Svetlana Lutsenko
Mar 5, 2009·Physical Review Letters·Yoshinori NishinoKazuhiro Maeshima
Jul 17, 2009·Journal of the Royal Society, Interface·Richard OrtegaAsunción Carmona
Jul 29, 2009·International Journal of Radiation Biology·Tatjana PauneskuGayle E Woloschak
Mar 1, 2009·Journal of Electron Spectroscopy and Related Phenomena·M R HowellsD Starodub
Aug 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Martin D de JongeStefan Vogt
Dec 24, 2010·Journal of Microscopy·D VanheckeV Lučić
Jul 16, 2011·Inorganic Chemistry·Drew Gorman-LewisMark P Jensen
Aug 30, 2011·Journal of Structural Biology·Minna R KrejciDerk Joester
Dec 14, 2011·Cancer Research·Hans C AroraGayle E Woloschak
Dec 21, 2011·Journal of Structural Biology·S BohicJ Susini
Mar 27, 2012·Analytica Chimica Acta·Mark P JensenGayle E Woloschak
Oct 20, 2012·Analytical and Bioanalytical Chemistry·Stefan Vogt, Martina Ralle
Oct 25, 2012·Journal of Synchrotron Radiation·Robert P WinarskiJörg Maser

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Citations

Jul 16, 2014·Analytical and Bioanalytical Chemistry·Stéphane RoudeauRichard Ortega
Dec 17, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fahmida JabeenGuenther K Bonn
Feb 29, 2016·Micron : the International Research and Review Journal for Microscopy·Marcel Menezes Lyra da CunhaSergio Marco
Feb 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Junjing DengChris J Jacobsen
Dec 25, 2015·Journal of Synchrotron Radiation·Gema Martínez-CriadoJohn Morse
Aug 19, 2015·ACS Chemical Neuroscience·Tanja DučićPaul Lingor
May 15, 2015·Optics Express·Doǧa GürsoyFrancesco De Carlo
Jan 22, 2015·Optics Express·Youssef S G NashedChris Jacobsen
Sep 2, 2014·Journal of Synchrotron Radiation·Adam P Hitchcock, Michael F Toney
Feb 24, 2016·Chemical Communications : Chem Comm·Dominic J Hare, Elizabeth J New
Dec 25, 2016·Chemistry : a European Journal·Carlos Sanchez-CanoPeter J Sadler
Oct 11, 2017·The New Phytologist·Antony van der EntJolanta Mesjasz-Przybyłowicz
Nov 20, 2016·Nature Communications·Fucai ZhangIan K Robinson
Feb 9, 2018·Scientific Reports·Xiaogang YangDoğa Gürsoy
Mar 12, 2019·Journal of Synchrotron Radiation·Michal OdstrcilMirko Holler
Aug 24, 2019·Scientific Reports·Darren J BateyChristoph Rau
Sep 2, 2017·Journal of Synchrotron Radiation·Emmanuel EsteveDominique Bazin
Jun 21, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yifeng LiaoSpiro Megremis
Dec 22, 2015·Nature Communications·Andrew C WardenVictoria S Haritos
May 3, 2018·The Review of Scientific Instruments·M HollerO Bunk
Mar 16, 2019·Cellular and Molecular Neurobiology·Jelena PopovićGayle E Woloschak
Mar 16, 2018·Nano Research·Koshonna BrownGayle E Woloschak
Jul 29, 2017·Physical Review. B·Junjing DengChris Jacobsen
Sep 2, 2019·The Review of Scientific Instruments·Junjing DengStefan Vogt
Oct 28, 2019·Metallomics : Integrated Biometal Science·Antony van der EntEmmanuelle Montargès-Pelletier
Jan 1, 2019·Pure and Applied Chemistry. Chimie Pure Et Appliquée·Roberto TerzanoKoen Janssens
Dec 3, 2017·The Review of Scientific Instruments·M HollerM Vitins
Mar 3, 2021·ACS Nano·Carlos Sanchez-CanoWolfgang J Parak

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Methods Mentioned

BETA
X-ray
fluorescence tomography

Software Mentioned

MAPS
EPICS
Delta
Windows
VxWorks
VxWorks IOC
Windows IOC
Xradia

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