PMID: 9537760Apr 16, 1998Paper

The biosynthesis and processing of vitellogenin in the fat bodies of females and males of the cockroach Leucophaea maderae

Insect Biochemistry and Molecular Biology
G Don-Wheeler, F Engelmann

Abstract

The juvenile hormone analog (JHA) methoprene was used to induce the synthesis of the yolk protein precursor vitellogenin (Vg) in adult females and males of the cockroach Leucophaea maderae. The female- and male-produced vitellogenin (VgF and VgM, respectively) contained polypeptides of 112, 95, 92, and 54 kDa. Also present in the secreted vitellogenins was a soffmall quantity of a short-lived transitional 155 kDa Vg polypeptide, and a variable amount of an 85 dDa species. Quantitatively, the VgF and VgM were significantly different in the Vg112 and Vg95 units (VgF > VgM), and in the Vg85 polypeptide (VgF < VgM). In the present study, the biosynthesis of Vg precursors in the fat bodies of females and males was examined using a short radiopulse with 35S-methionine/cysteine and 32P-orthophosphate. The glycosylation of the Vg precursors was examined by digestion with endoglycosidase H and by the inhibition of N-linked glycosylation with tunicamycin. The data showed that in both females and males, the synthesis of the vitellogenin precursor occurred in a stepwise fashion: (1) the co-translational glycosylation of Vg203; (2) the post-translational phosphorylation of Vg203 to form Vg220; (3) the proteolytic processing of Vg220 to form...Continue Reading

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Citations

Jun 5, 2002·Insect Biochemistry and Molecular Biology·Ana Paula SalernoHatisaburo Masuda
Jan 26, 2000·Insect Biochemistry and Molecular Biology·J M LeeK Oishi
Jan 29, 2011·The Journal of Experimental Biology·Heli HavukainenGro V Amdam
May 11, 2012·The Journal of Experimental Biology·Heli HavukainenØyvind Halskau
Sep 16, 2008·Journal of Insect Physiology·Muhammad Tufail, Makio Takeda
Nov 15, 2007·Archives of Insect Biochemistry and Physiology·Muhammad TufailMakio Takeda
Mar 6, 2012·Developmental and Comparative Immunology·Shicui ZhangHongmiao Wang
Apr 7, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·S RomeroM Cunningham
Jul 28, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Françoise Goudey-PerrièreClaude Perrière

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