PMID: 5257134Jun 1, 1969Paper

The biosynthesis of ferredoxin in a cell-free system

Proceedings of the National Academy of Sciences of the United States of America
A C Trakatellis, G Schwartz

Abstract

The investigation reported in this communication was concerned with the biosynthesis of ferredoxin in a cell-free system. A cell-free system, with the ability to incorporate amino acids into peptides and proteins, was developed from Cl. pasteurianum, and its requirements were established. After the incorporation of L-alanine-C(14), ferredoxin-C(14) was isolated in crystalline form with the aid of added carrier ferredoxin. Amino acid analyses and absorption spectra strongly indicated that the isolated ferredoxin-C(14), crystallized three times, was essentially free from impurities. Furthermore, experiments with Fe(59) strongly indicated that the iron may combine with the apoprotein after the latter is completely synthesized and released from the polysomes.

References

Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W Lovenberg, B E Sobel
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A C Trakatellis
Jul 27, 1964·Biochemical and Biophysical Research Communications·M TanakaK T Yasunobu
Mar 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·B B BUCHANANJ C RABINOWITZ
Aug 1, 1963·Biochemical and Biophysical Research Communications·R C VALENTINER D SAGERS
Sep 12, 1964·Nature·A C TRAKATELLISM MONTJAR

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Citations

Jan 1, 1979·Planta·J G HuismanA Bernards
Jul 29, 1980·Biochimica Et Biophysica Acta·H H Liao, J C Rabinowitz
Mar 30, 2006·Biotechnology and Bioengineering·Marcus E BoyerJames R Swartz
Dec 1, 1974·Journal of Bacteriology·L Legault-Demare, G H Chambliss

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