PMID: 2311Feb 13, 1976

The biosynthesis of multi-L-arginyl-poly(L-aspartic acid) in the filamentous cyanobacterium Anabaena cylindrica

Biochimica Et Biophysica Acta
R D Simon

Abstract

The cyanobacteria produce multi-L-arginyl-poly (aspartic acid), a high molecular weight (Mr=25 000-125 000) branched polypeptide consisting of a poly(aspartic acid) core with L-arginyl residues peptide bonded to each free carboxyl group of the poly(aspartic acid). An enzyme which will elongate Arg-poly(Asp) has been isolated and purified 92-fold from the filamentous cyanobacterium Anabaena cylindrica. The enzyme incorporates arginine and aspartic acid into Arg-poly(Asp) in a reaction which requires ATP, KCl, MgCl2, and a sulfhydryl reagent. The enzymatic incorporation of arginine is dependent upon the presence of L-aspartic acid but not visa versa, a finding which suggests the order of amino acid addition to the branched polypeptide-aspartic acid is added to the core followed by the attachment of an arginine branch. The elongation of Arg-poly(Asp) in-vitro is insensitive to the addition of protein synthesis inhibitors and to the addition of nucleases. These findings support the notion previosly suggested from in-vivo studies that Arg-poly(Asp) is synthesized via a non-ribosomal route and also demonstrate that amino-acetylated transfer-RNAs play no part in at least one step of the biosynthetic mechanism.

References

Mar 5, 1971·Nature·T S StewartJ L Strominger
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R D Simon

Citations

Sep 19, 2007·Applied Microbiology and Biotechnology·Hans MooibroekAlexander Steinbüchel
Aug 6, 2008·Applied Microbiology and Biotechnology·Toshinobu Arai, Kuniki Kino
Oct 13, 2010·Applied Microbiology and Biotechnology·Yasser Abd-El-KaremAlexander Steinbüchel
Apr 13, 2013·Applied Microbiology and Biotechnology·Jikun DuShining Zhou
Sep 14, 2006·Photosynthesis Research·Francisco J FlorencioMarika Lindahl
Apr 15, 2008·Applied and Environmental Microbiology·Anna SteinleAlexander Steinbüchel
Nov 4, 2008·Applied and Environmental Microbiology·Ahmed SallamAlexander Steinbüchel
Sep 4, 2012·Applied and Environmental Microbiology·Steffen LütteBärbel Friedrich
Jul 27, 2001·Applied and Environmental Microbiology·J R TrzebiatowskiF J de Bruijn
Mar 28, 2002·Applied and Environmental Microbiology·Lauren L WingardA Michelle Wood
Jun 29, 2002·Applied and Environmental Microbiology·Kay FreyAlexander Steinbüchel
Dec 8, 2005·Applied and Environmental Microbiology·Yasser ElbahloulAlexander Steinbüchel
Feb 5, 2005·Applied and Environmental Microbiology·Yasser ElbahloulAlexander Steinbüchel
Feb 8, 2006·Applied and Environmental Microbiology·Yasser Elbahloul, Alexander Steinbüchel
Jan 25, 2011·Microbial Biotechnology·Jens KrollAlexander Steinbüchel
Nov 27, 2015·Microbial Cell Factories·Björn WatzerKarl Forchhammer
Feb 11, 2014·Applied and Environmental Microbiology·Maja FrommeyerAlexander Steinbüchel
Jun 29, 2010·Nature Reviews. Microbiology·Bernd H A Rehm
Oct 22, 2009·Plant Biotechnology Journal·Maja HühnsInge Broer
Aug 19, 2018·Applied and Environmental Microbiology·Björn Watzer, Karl Forchhammer

Related Concepts

Cyanobacteria
Arginine hydrochloride
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Hydrogen-Ion Concentration
Magnesium
Peptide Biosynthesis
Peptide synthase
Potassium
Sulfhydryl Reagents

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