The Biosynthetic Gene Cluster for Andrastin A in Penicillium roqueforti

Frontiers in Microbiology
Juan F Rojas-AedoRenato Chávez

Abstract

Penicillium roqueforti is a filamentous fungus involved in the ripening of several kinds of blue cheeses. In addition, this fungus produces several secondary metabolites, including the meroterpenoid compound andrastin A, a promising antitumoral compound. However, to date the genomic cluster responsible for the biosynthesis of this compound in P. roqueforti has not been described. In this work, we have sequenced and annotated a genomic region of approximately 29.4 kbp (named the adr gene cluster) that is involved in the biosynthesis of andrastin A in P. roqueforti. This region contains ten genes, named adrA, adrC, adrD, adrE, adrF, adrG, adrH, adrI, adrJ and adrK. Interestingly, the adrB gene previously found in the adr cluster from P. chrysogenum, was found as a residual pseudogene in the adr cluster from P. roqueforti. RNA-mediated gene silencing of each of the ten genes resulted in significant reductions in andrastin A production, confirming that all of them are involved in the biosynthesis of this compound. Of particular interest was the adrC gene, encoding for a major facilitator superfamily transporter. According to our results, this gene is required for the production of andrastin A but does not have any role in its secre...Continue Reading

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Citations

Oct 2, 2020·Natural Product Reports·Lena Barra, Ikuro Abe
Dec 23, 2017·Frontiers in Microbiology·Claudia TorrentRenato Chávez
Dec 14, 2018·Frontiers in Microbiology·Fernando Guzmán-ChávezArnold J M Driessen
Jan 2, 2019·Fungal biology and biotechnology·Sietske GrijseelsRasmus John Normand Frandsen
Dec 16, 2018·International Journal of Food Microbiology·Monica OlsenMichael Sulyok

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PCR

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