PMID: 7084564Apr 1, 1982Paper

The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera)

Molecular and Cellular Endocrinology
C HetruD H Horn

Abstract

Ovaries of adult females of Locusta migratoria synthesize impressive amounts of the steroid hormone ecdysone (and related ecdysteroids) during the late phases of vitellogenesis. The present study, aimed at elucidating the sequence of the biosynthetic steps that lead from cholesterol to ecdysone, has taken benefit of this remarkable biological model by using a double approach: (1) isolation and physico-chemical identification of endogenous biogenetic intermediates; (2) metabolic study of labelled putative precursor molecules. The data presented in this paper lead us to propose the following sequence of events: conversion of cholesterol to 3 beta-hydroxy-5 beta-cholest-7-en-6-one (via several intermediates not identified in this study) followed by 14 beta-hydroxylation to 3 beta, 14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; hydroxylation on the side-chain at C-25 and C-22 (in this order) to 2-deoxyecdysone; hydroxylation at C-2 to ecdysone.

Citations

May 1, 1986·Molecular and Cellular Endocrinology·F LachaiseR Lafont
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Agosin, J Srivatsan
Jan 1, 1993·Insect Biochemistry and Molecular Biology·M L GrieneisenLawrence I Gilbert
Apr 5, 2005·Insect Biochemistry and Molecular Biology·Douglas H SieglaffMark R Brown
Aug 6, 2008·Annual Review of Entomology·Mark R BrownH H Rees
Apr 25, 2012·Biochemical and Biophysical Research Communications·Hajime OnoRitsuo Nishida
Nov 1, 1994·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·J P Roussel
Dec 2, 2011·Médecine sciences : M/S·Evelyne Jouvin Marche
Jan 1, 1992·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·J P Roussel
Jan 1, 1994·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·J P Roussel

Related Concepts

High Pressure Liquid Chromatography Procedure
Ecdysone
Chromatography, Gas-Liquid-Mass Spectrometry
Caelifera
Ovary

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