The biotechnology (genetic transformation and molecular biology) of Bixa orellana L. (achiote)

Planta
Jaime A Teixeira da SilvaRenata Rivera-Madrid

Abstract

Genetic transformation allows for greater bixin or norbixin production in achiote. Knowledge of genes that control the biosynthesis of these important secondary metabolites will allow for targeted amplification in transgenic plants. Annatto is a natural dye or coloring agent derived from the seeds, or their arils, of achiote (Bixa orellana L.), and is commercially known as E160b. The main active component of annatto dye is water-insoluble bixin, although water-soluble norbixin also has commercial applications. Relative to other antioxidants, bixin is light- and temperature stable and is thus safe for human consumption. Bixin is, therefore, widely applied as a dye and as an antioxidant in the medico-pharmaceutical, food, cosmetic, and dye industries. Even though bixin has also been isolated from leaves and bark, yield is lower than from seeds. More biotechnology-based research of this industrial and medicinal plant is needed. Building on provisional genetic transformation studies, it would be advantageous to transform genes that could result in greater bixin or norbixin production. Reliable protocols for the extraction of bixin and norbixin, as well as deeper knowledge of the genes that control the biosynthesis of these importan...Continue Reading

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Citations

Jun 4, 2020·Acta Cirúrgica Brasileira·Lízia Daniela E Silva NascimentoVicente Galber Freitas Viana
Apr 16, 2020·Photodiagnosis and Photodynamic Therapy·Marcela Leticia Leal GonçalvesSandra Kalil Bussadori
Jul 17, 2021·Journal of Biotechnology·Leepica Kapoor, Siva Ramamoorthy
Jul 29, 2021·Plant Science : an International Journal of Experimental Plant Biology·Ressin VargheseSiva Ramamoorthy

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Methods Mentioned

BETA
transgenic
PCR

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