The blood-testis barrier in the toad (Bufo arenarum Hensel): a freeze-fracture and lanthanum tracer study

The Anatomical Record
J C Cavicchia, G A Moviglia

Abstract

Intercellular junctions between Sertoli cells in the toad testis were studied by freeze-fracture and electron-opaque intercellular markers. These junctional specializations are characterized in thin sections by a series of focal fusions on the outer leaflets of both adjacent cell plasmalemmas, associated with bundles of fine filaments in the subjacent Sertoli cell cytoplasms. However, the wide subsurface cisterna of the endoplasmic reticulum, a component constantly associated with Sertoli cell junctions in mammals, is absent in the toad. The intravascularly injected lanthanum hydroxide, used as a tracer compound, gains access to the seminiferous tubules and surrounds spermatogonia and leptotene spermatocytes, but is persistently excluded from germ cells in later stages of development. This indicates that, as is the case in the mammalian testis, a permeability barrier to lanthanum is established which isolates all germ cells beyond leptotene spermatocytes. Freeze-fracture reveals the characteristic occluding junctions between Sertoli cells, but a variation in their geometric patterns was clearly observed in different regions of the toad seminiferous epithelium. The membrane-fractured faces of Sertoli cells embracing differentiat...Continue Reading

References

Oct 1, 1977·Biology of Reproduction·M Dym, J C Cavicchia
Dec 1, 1977·Journal of Ultrastructure Research·R MeyerD McGinley
Dec 1, 1975·Journal of Ultrastructure Research·E RealeS Iurato
Mar 1, 1976·The Journal of Experimental Zoology·M R Kalt
Aug 1, 1973·The Journal of Cell Biology·P Claude, D A Goodenough
Jan 1, 1974·The Journal of Cell Biology·J B Wade, M J Karnovsky
Nov 1, 1972·Journal of Ultrastructure Research·S C Reed, H P Stanley
Jun 1, 1967·The Journal of Cell Biology·J P Revel, M J Karnovsky
Aug 1, 1982·The Anatomical Record·J C Cavicchia, G A Moviglia
May 25, 1956·The Journal of Biophysical and Biochemical Cytology·M H BURGOS, D W FAWCETT
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

❮ Previous
Next ❯

Citations

Nov 1, 2011·Zoological Science·Lia Raquel de Souza SantosClassius de Oliveira
Nov 23, 2012·Zygote : the Biology of Gametes and Early Embryos·Ana Lucrecia Iruzubieta VillagraSilvia Nélida Fernández
May 9, 2008·Micron : the International Research and Review Journal for Microscopy·Lia Raquel de Souza Santos, Classius de Oliveira
May 1, 1996·Ultrastructural Pathology·J C CavicchiaL Ortiz
Apr 19, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Alfonsina MoralesJuan Carlos Cavicchia
Jan 1, 1992·Microscopy Research and Technique·R M Pelletier, S W Byers
Jan 1, 1992·Microscopy Research and Technique·J C CavicchiaL A Gutierrez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.