The blue light-dependent phosphorylation of the CCE domain determines the photosensitivity of Arabidopsis CRY2

Molecular Plant
Qin WangChentao Lin

Abstract

Arabidopsis cryptochrome 2 (CRY2) is a blue light receptor that mediates light inhibition of hypocotyl elongation and long-day promotion of floral initiation. CRY2 is known to undergo blue light-dependent phosphorylation, which is believed to serve regulatory roles in the function of CRY2. We report here on a biochemical and genetics study of CRY2 phosphorylation. Using mass spectrometry analysis, we identified three serine residues in the CCE domain of CRY2 (S598, S599, and S605) that undergo blue light-dependent phosphorylation in Arabidopsis seedlings. A study of serine-substitution mutations in the CCE domain of CRY2 demonstrates that CRY2 contains two types of phosphorylation in the CCE domain, one in the serine cluster that causes electrophoretic mobility upshift and the other outside the serine cluster that does not seem to cause mobility upshift. We showed that mutations in the serine residues within and outside the serine cluster diminished blue light-dependent CRY2 phosphorylation, degradation, and physiological activities. These results support the hypothesis that blue light-dependent phosphorylation of the CCE domain determines the photosensitivity of Arabidopsis CRY2.

Citations

Nov 16, 2016·Science·Qin WangChentao Lin
Nov 20, 2016·Photochemistry and Photobiology·Zhaohe YangYoshito Oka
Mar 23, 2017·The Plant Journal : for Cell and Molecular Biology·Yuyu ZhengZiqiang Zhu
Jun 24, 2017·Cellular and Molecular Life Sciences : CMLS·Alejandra A CovarrubiasCaspar C C Chater
Nov 16, 2017·The New Phytologist·Qin WangChentao Lin
Jul 26, 2018·The Plant Journal : for Cell and Molecular Biology·Maike EckelAlfred Batschauer
Jan 27, 2016·Journal of Plant Research·Bobin LiuYoshito Oka
Mar 15, 2020·Annual Review of Plant Biology·Qin Wang, Chentao Lin
Feb 18, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M HammadM Ahmad
Jul 11, 2020·Scientific Reports·Maria AlbaqamiCarlos F Martino
Nov 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yangyang ZhouJigang Li
Mar 25, 2020·International Journal of Molecular Sciences·Arndt Wallmann, Christopher Kesten
May 14, 2020·Nature Structural & Molecular Biology·Ling MaPing Yin
Jan 2, 2021·Genomics, Proteomics & Bioinformatics·Zhi-Fang GaoBai-Chen Wang
Apr 5, 2019·Seminars in Cell & Developmental Biology·Inyup Paik, Enamul Huq
Jul 17, 2016·Current Opinion in Plant Biology·Margaret Ahmad
Apr 14, 2021·Nature Communications·Yadi ChenChentao Lin
Oct 16, 2021·Nature Plants·Xu WangChentao Lin

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