The bone anabolic effects of irisin are through preferential stimulation of aerobic glycolysis

Bone
Dongdong ZhangSung-Kil Lim

Abstract

Irisin, a recently identified hormone secreted by skeletal muscle in response to exercise, exhibits anabolic effects on the skeleton primarily through the stimulation of bone formation. However, the mechanism underlying the irisin-stimulated anabolic response remains largely unknown. To uncover the underlying mechanism, we biosynthesized recombinant irisin (r-irisin) using an Escherichia coli expression system and used it to treat several osteoblast cell types. Our synthesized r-irisin could promote proliferation and differentiation of osteoblasts as evidenced by enhanced expression of osteoblast-specific transcriptional factors, including Runt-related transcription factor-2 (Runx2), Oster (Osx), as well as early osteoblastic differentiation markers such as alkaline phosphatase (Alp) and collagen type I alpha 1 (Col1a1). Furthermore, we showed that the promotion of r-irisin on the proliferation and differentiation of osteoblast lineage cells are preferentially through aerobic glycolysis, as indicated by the enhanced abundance of representative enzymes such as lactate dehydrogenase A (LDHA) and pyruvate dehydrogenase kinase 1 (PDK1), together with increased lactate levels. Suppression of r-irisin-mediated aerobic glycolysis with...Continue Reading

Citations

Feb 14, 2019·The Journal of Clinical Endocrinology and Metabolism·Andrea PalermoMaria Grano
Aug 14, 2019·Menopause : the Journal of the North American Menopause Society·Kunyan ZhouDan Shan
Jun 24, 2020·Journal of Cellular Physiology·Xiaofang ZhuJake J Chen
Aug 25, 2019·Medicina·Paulina KortaAgnieszka Mazur-Biały
Nov 7, 2019·Menopause : the Journal of the North American Menopause Society·Kunyan ZhouDan Shan
May 20, 2021·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Francesco PuccettiDonald E Low
May 22, 2021·Frontiers in Cell and Developmental Biology·Lifei LiuJun Zou
Aug 17, 2021·Frontiers in Cardiovascular Medicine·Wei ZhaoJianhui Rong

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