The Bordetella bronchiseptica nic locus encodes a nicotinic acid degradation pathway and the 6-hydroxynicotinate-responsive regulator BpsR

Molecular Microbiology
Timothy J Brickman, Sandra K Armstrong

Abstract

The classical Bordetella species use amino acids as carbon sources and can catabolize organic acids and tricarboxylic acid cycle intermediates. They are also auxotrophic for nicotinamide adenine dinucleotide (NAD) pathway precursors such as nicotinic acid. Bordetellae have a putative nicotinate catabolism gene locus highly similar to that characterized in Pseudomonas putida KT2440. This study determined the distribution of the nic genes among Bordetella species and analyzed the regulation of this nicotinic acid degradation system. Transcription of the Bordetella bronchiseptica nicC gene was repressed by the NicR ortholog, BpsR, previously shown to regulate extracellular polysaccharide synthesis genes. nicC expression was derepressed by nicotinic acid or by the first product of the degradation pathway, 6-hydroxynicotinic acid, which was shown to be the inducer. Results using mutants with either a hyperactivated pathway or an inactivated pathway showed a marked effect on growth on nicotinic acid that indicated this degradation pathway influences NAD biosynthesis. Pathway dysregulation also affected Bordetella BvgAS-mediated virulence gene regulation, demonstrating that fluctuation of intracellular nicotinic acid pools impacts Bvg...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Feb 1, 1992·Journal of Bacteriology·J F MillerS Falkow
Oct 1, 1970·Journal of General Microbiology·D W Stainer, M J Scholte
Nov 1, 1982·Infection and Immunity·D R Schneider, C D Parker
Mar 1, 1993·Infection and Immunity·A R Melton, A A Weiss
Sep 15, 1998·Journal of Medical Microbiology·B J Plotkin, D A Bemis
Dec 20, 1999·Journal of Biotechnology·M ThalenJ Tramper
Dec 1, 1953·Japanese Journal of Medical Science & Biology·H FUKUMIT UCHIDA
Mar 1, 1960·The Journal of Hygiene·B W LACEY
Aug 20, 2004·Journal of Bacteriology·Yasuhiko IrieMing H Yuk
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Feb 18, 2006·Journal of Bacteriology·C A CummingsJ F Miller
Jun 26, 2007·Journal of Bacteriology·Gina Parise SloanRajendar Deora
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·José I JiménezEduardo Díaz
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Nov 13, 2009·Nucleic Acids Research·Paramvir S DehalAdam P Arkin
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Julien HerrouFrançoise Jacob-Dubuisson
Apr 1, 2011·Environmental Microbiology·José Ignacio JiménezEduardo Díaz
Oct 4, 2011·Nature Reviews. Microbiology·Georg FuchsJohann Heider
Nov 6, 2012·Current Opinion in Biotechnology·Eduardo DíazJuan Nogales
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg
Mar 13, 2014·Nature Reviews. Microbiology·Jeffrey A MelvinPeggy A Cotter
Apr 4, 2015·Critical Reviews in Biochemistry and Molecular Biology·Andrey NikiforovMathias Ziegler
Oct 27, 2016·Molecular Microbiology·Timothy J BrickmanSandra K Armstrong
Aug 27, 2017·Applied and Environmental Microbiology·Filipe Branco Dos SantosPhilippe Goffin

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