PMID: 9525645Apr 3, 1998Paper

The bovine leukemia virus encapsidation signal is composed of RNA secondary structures

Journal of Virology
Louis M Mansky, R M Wisniewski

Abstract

The encapsidation signal of bovine leukemia virus (BLV) was previously shown by deletion analysis to be discontinuous and to extend into the 5' end of the gag gene (L. Mansky et al., J. Virol. 69:3282-3289, 1995). The global minimum-energy optimal folding for the entire BLV RNA, including the previously mapped primary and secondary encapsidation signal regions, was analyzed. Two stable stem-loop structures (located just downstream of the gag start codon) were predicted within the primary signal region, and one stable stem-loop structure (in the gag gene) was predicted in the secondary signal region. Based on these predicted structures, we introduced a series of mutations into the primary and secondary encapsidation signals in order to explore the sequence and structural information contained within these regions. The replication efficiency and levels of cytoplasmic and virion RNA were analyzed for these mutants. Mutations that disrupted either or both of the predicted stem-loop structures of the primary signal reduced the replication efficiency by factors of 7 and 40, respectively; similar reductions in RNA encapsidation efficiency were observed. The mutant with both stem-loop structures disrupted had a phenotype similar to tha...Continue Reading

References

May 11, 1992·Journal of Virology·G L Buchschacher, A T Panganiban
Jun 1, 1992·Virology·T HayashiH Shibuta
Jan 1, 1991·Journal of Virology·R Aronoff, M Linial
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M FreierD H Turner
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A JaegerM Zuker
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K ShimotohnoT Sugimura
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N SagataY Ikawa
May 1, 1987·Journal of Virology·D ArmentanoE Gilboa
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Osborne, A D Miller
Mar 15, 1968·International Journal of Cancer. Journal International Du Cancer·H M Temin
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M SeikiM Yoshida
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Watanabe, H M Temin
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I S ChenD W Golde
Jun 1, 1984·Molecular and Cellular Biology·S Kawai, M Nishizawa
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W I Sundquist, S Heaphy

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Citations

Jul 23, 2002·Journal of Virology·Huating WangLouis M Mansky
Jul 4, 2012·Chembiochem : a European Journal of Chemical Biology·Shuang GaoZhen Xi
Oct 12, 2016·Viruses·Lizna M AliFarah Mustafa
Jun 12, 2014·Frontiers in Microbiology·Ayna Alfadhli, Eric Barklis
Jun 7, 2002·Biophysical Chemistry·David R MorcockJosé R Casas-Finet
Jul 19, 2011·Journal of Molecular Biology·Leslie J Parent, Nicole Gudleski

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