The Bovine Metabolome

Metabolites
Aidin ForoutanDavid S Wishart

Abstract

From an animal health perspective, relatively little is known about the typical or healthy ranges of concentrations for many metabolites in bovine biofluids and tissues. Here, we describe the results of a comprehensive, quantitative metabolomic characterization of six bovine biofluids and tissues, including serum, ruminal fluid, liver, Longissimus thoracis (LT) muscle, semimembranosus (SM) muscle, and testis tissues. Using nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and inductively coupled plasma-mass spectrometry (ICP-MS), we were able to identify and quantify more than 145 metabolites in each of these biofluids/tissues. Combining these results with previous work done by our team on other bovine biofluids, as well as previously published literature values for other bovine tissues and biofluids, we were able to generate quantitative reference concentration data for 2100 unique metabolites across five different bovine biofluids and seven different tissues. These experimental data were combined with computer-aided, genome-scale metabolite inference techniques to add another 48,628 unique metabolites that are biochemically expected to be in bovine tissues or biofluids. ...Continue Reading

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Citations

May 16, 2021·Reproductive Biology·Coline MahéMarie Saint-Dizier
Jun 3, 2021·Foods·Abdul-Hamid M EmwasJoanna Izabela Lachowicz
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Marta LombóMaría-Jesús Sánchez-Calabuig
Sep 15, 2021·The Journal of Nutritional Biochemistry·Bharath S AvirineniPrasanth K Chelikani
Oct 16, 2021·Cell Systems·Sylvie EstrelaÁlvaro Sánchez
Jan 9, 2022·Scientific Reports·Virginia M ArtegoitiaH C Freetly

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
pull-down
ELISA
metabolomics assay

Software Mentioned

MySQL
Analyst
PolySearch2
DataWrangler
PubMed
PolySearch
ScienceDirect
Chenomx NMR Suite
MetaboCard
BMDB

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