The bovine parainfluenza virus type-3 (BPIV-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental BPIV-3 challenge

Vaccine
E J HaanesR Wardley

Abstract

Despite the availability of numerous vaccine schedules, "shipping fever", an acute bronchopneumonia brought on in part by a complex of bovine respiratory viruses, remains a major source of economic loss in the beef and dairy industries. We are exploring new strategies of bovine vaccine design which we hope may provide more effective and more cost-efficient control of these pathogens. In this report, we examined the possible use of subunit vaccines, using as an example the hemagglutinin/neuraminidase (HN) protein of bovine parainfluenza virus type-3 (BPIV-3) expressed in the baculovirus expression system. We showed that the protein was expressed at high levels, and was modified to a similar, but not identical size as the native HN protein expressed from BPIV-3 infected bovine cells. We further demonstrated antigenicity and biological activity of the expressed HN protein. Finally, we vaccinated colostrum deprived sera-negative calves with the baculo HN recombinant protein and challenged with BPIV-3. Vaccination induced excellent serum neutralizing antibody responses, and surprisingly, good mucosal antibody responses, even though the vaccine was administered parenterally. The vaccinated animals were well protected against challenge.

References

Jan 1, 1993·Vaccine·S van Drunen Littel-van den HurkT J Zamb
Jan 1, 1993·Veterinary Research Communications·S Belák, A Ballagi-Pordány

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Citations

Jun 19, 2008·The Journal of General Virology·Paul Francis HorwoodTimothy John Mahony
Mar 26, 1998·Plasmid·M T Dertzbaugh
Sep 26, 2006·Advances in Virus Research·Monique M van Oers
Jul 28, 2016·Journal of Virological Methods·Jizong LiJieyuan Jiang
Jul 6, 2017·Journal of Immunoassay & Immunochemistry·Theophilus Aghogho Jarikre, Benjamin Obukowho Emikpe
Jun 16, 2019·Veterinary Sciences·Harun AlbayrakManfred Weidmann
Apr 21, 2017·International Journal of Veterinary Science and Medicine·Nader M SobhySagar M Goyal
Apr 14, 2018·Frontiers in Cellular and Infection Microbiology·Jizong LiJieyuan Jiang
Jul 18, 2021·Environmental Science and Pollution Research International·AbdulRahman A SaiedMohie A M Haridy

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