The box VII motif of Escherichia coli DnaA protein is required for DnaA oligomerization at the E. coli replication origin.

The Journal of Biological Chemistry
Magdalena M Felczak, Jon M Kaguni

Abstract

Escherichia coli DnaA protein initiates DNA replication from the chromosomal origin, oriC, and regulates the frequency of this process. Structure-function studies indicate that the replication initiator comprises four domains. Based on the structural similarity of Aquifex aeolicus DnaA to other AAA+ proteins that are oligomeric, it was proposed that Domain III functions in oligomerization at oriC (Erzberger, J. P., Pirruccello, M. M., and Berger, J. M. (2002) EMBO J. 21, 4763-4773). Because the Box VII motif within Domain III is conserved among DnaA homologues and may function in oligomerization, we substituted conserved Box VII amino acids of E. coli DnaA with alanine by site-directed mutagenesis to examine the role of this motif. All mutant proteins are inactive in initiation from oriC in vivo and in vitro, but they support RK2 plasmid DNA replication in vivo. Thus, RK2 requires only a subset of DnaA functions for plasmid DNA replication. Biochemical studies on a mutant DnaA carrying an alanine substitution at arginine 281 (R281A) in Box VII show that it is inactive in in vitro replication of an oriC plasmid, but this defect is not from the failure to bind to ATP, DnaB in the DnaB-DnaC complex, or oriC. Because the mutant Dna...Continue Reading

References

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R S FullerA Kornberg
Jul 19, 1996·The Journal of Biological Chemistry·C Margulies, J M Kaguni
Aug 30, 1996·The Journal of Biological Chemistry·K Tougu, K J Marians
Feb 1, 1997·Analytical Biochemistry·K M Vossen, M G Fried
Sep 12, 1997·The Journal of Biological Chemistry·M D Sutton, J M Kaguni
Sep 23, 1997·Journal of Molecular Biology·M D Sutton, J M Kaguni
Jan 7, 1998·Journal of Molecular Biology·M D Sutton, J M Kaguni
Jun 5, 1998·Microbiology·Dagmara JakimowiczJolanta Zakrzewska-Czerwinńska
Jun 9, 2000·Molecular Microbiology·F BlaesingW Messer
Sep 12, 2001·The Journal of Biological Chemistry·K M Carr, J M Kaguni
Mar 27, 2002·Genes & Development·Stephen P Bell
Jun 18, 2002·The EMBO Journal·Megan J DaveyMike O'Donnell
Aug 6, 2002·The Journal of Biological Chemistry·Kevin M Carr, Jon M Kaguni
Apr 12, 2003·Nucleic Acids Research·Norie FujikawaShigeyuki Yokoyama
Oct 20, 2004·Molecular Microbiology·Nilangshu Das, Dhruba K Chattoraj

❮ Previous
Next ❯

Citations

Apr 17, 2007·Nature Reviews. Microbiology·Melissa L Mott, James M Berger
Feb 17, 2010·Nature Reviews. Microbiology·Tsutomu KatayamaKazuyuki Fujimitsu
Jul 11, 2006·Nature Structural & Molecular Biology·Jan P ErzbergerJames M Berger
Oct 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Diana T MillerAlan C Leonard
Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kazutoshi Kasho, Tsutomu Katayama
Jul 28, 2009·The Journal of Biological Chemistry·Kenji KeyamuraTsutomu Katayama
Aug 13, 2011·The Journal of Biological Chemistry·Rahul SaxenaElliott Crooke
Feb 4, 2011·Nucleic Acids Research·Sundari ChodavarapuJon M Kaguni
Aug 31, 2013·Nucleic Acids Research·Sundari ChodavarapuJon M Kaguni
Sep 18, 2007·Applied and Environmental Microbiology·Katarzyna Hupert-KocurekJon M Kaguni
Feb 5, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hironori Kawakami, Tsutomu Katayama
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Jan P Erzberger, James M Berger
Jun 7, 2006·Annual Review of Microbiology·Jon M Kaguni
Jun 12, 2013·Annual Review of Biochemistry·Alessandro CostaJames M Berger
Jun 7, 2011·Annual Review of Microbiology·Alan C Leonard, Julia E Grimwade
Aug 23, 2011·Current Opinion in Chemical Biology·Jon M Kaguni
Jan 9, 2008·Molecular Microbiology·Sundari ChodavarapuJon M Kaguni
Oct 21, 2015·Frontiers in Microbiology·Chengcheng SongHe Huang
Jun 18, 2015·Frontiers in Microbiology·Alan C Leonard, Julia E Grimwade
Sep 15, 2012·Biochimie·Shawna M RotoliSubhasis B Biswas
Feb 5, 2010·Molecular Cell·Magdalena Makowska-Grzyska, Jon M Kaguni
Jul 13, 2016·Research in Microbiology·Juraj BugalaJozef Grones
Jan 10, 2018·Frontiers in Microbiology·Tsutomu KatayamaHironori Kawakami
Oct 4, 2011·Nature·Karl E DuderstadtJames M Berger
May 10, 2005·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Jan 25, 2008·The Journal of Biological Chemistry·Shogo OzakiTsutomu Katayama
Sep 27, 2019·Frontiers in Microbiology·Alan C LeonardJulia E Grimwade
May 20, 2005·The Journal of Biological Chemistry·Hironori KawakamiTsutomu Katayama
Jan 6, 2016·The Journal of Biological Chemistry·Magdalena M FelczakJon M Kaguni
Mar 16, 2018·Frontiers in Microbiology·Flemming G Hansen, Tove Atlung
Jan 23, 2008·Biochemical Society Transactions·Tsutomu Katayama
Dec 13, 2016·The Journal of Biological Chemistry·Kazutoshi KashoTsutomu Katayama

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.