PMID: 8955412Dec 1, 1996Paper

The Bradyrhizobium japonicum fegA gene encodes an iron-regulated outer membrane protein with similarity to hydroxamate-type siderophore receptors

Journal of Bacteriology
Kristin LeVier, Mary Lou Guerinot

Abstract

Iron is important in the symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, yet little is known about rhizobial iron acquisition strategies. Analysis of outer membrane proteins (OMPs) from B. japonicum 61A152 identified three iron-regulated OMPs in the size range of several known receptors for Fe(III)-scavenging siderophores. One of the iron-regulated proteins, FegA, was purified and microsequenced, and a reverse genetics approach was used to clone a fegA-containing DNA fragment. Sequencing of this fragment revealed a single open reading frame of 750 amino acids. A putative N-terminal signal sequence of 14 amino acids which would result in a mature protein of 736 amino acids with a molecular mass of 80,851 Da was predicted. FegA shares significant amino acid similarity with several Fe(III)-siderophore receptors from gram-negative bacteria and has greater than 50% amino acid similarity and 33% amino acid identity with two [corrected] bacterial receptors for hydroxamate-type Fe(III)-siderophores. A dendrogram describing total inferred sequence similarity among 36 TonB-dependent OMPs was constructed; FegA grouped with Fe(III)-hydroxamate receptors. The transcriptional start site of fegA was ma...Continue Reading

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Citations

Sep 20, 2005·Molecular Plant-microbe Interactions : MPMI·Heather P BensonMary Lou Guerinot
Jun 16, 2006·FEMS Microbiology Reviews·Gesine RudolphHans-Martin Fischer
Jan 31, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·William WrightRanjan Chakraborty
Nov 7, 1999·Molecular Plant-microbe Interactions : MPMI·K H YeomanA W Johnston
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Brent N KaiserDavid A Day
Jun 28, 2011·Journal of Bacteriology·Sandra K Small, Mark R O'Brian
Jan 31, 2018·Molecular Microbiology·Anushila Chatterjee, Mark R O'Brian
Mar 29, 2000·Journal of Bacteriology·M B RogersS B Calderwood
Feb 20, 2004·Journal of Bacteriology·Heather P BensonMary Lou Guerinot
Oct 4, 2006·BMC Bioinformatics·Pierre-Etienne JacquesRyszard Brzezinski
Oct 14, 2009·BMC Biology·Oliver MirusEnrico Schleiff

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