Apr 11, 2020

A general strategy to red-shift green fluorescent protein based biosensors

BioRxiv : the Preprint Server for Biology
S. Zhang, Hui-wang Ai

Abstract

Compared to green fluorescent protein (GFP) based biosensors, red fluorescent protein (RFP) based biosensors are inherently advantageous because of reduced phototoxicity, decreased autofluorescence, and enhanced tissue penetration. However, there is a limited choice of RFP-based biosensors and development of each biosensor requires significant effort. Herein, we describe a general and convenient method which uses the genetically encoded amino acid, 3-aminotyrosine (aY), to convert GFPs and GFP-based biosensors into red.

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Mentioned in this Paper

Computer Software
Study
Brain Injuries
Magnetic Resonance Imaging
Functional Neuroimaging
Diffusion Weighted Imaging
Brain
Neuroscience and Neuropsychiatric Research
FMRI
Neuroimaging

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