The brain in vivo expresses the 2',3'-cAMP-adenosine pathway.

Journal of Neurochemistry
Jonathan D VerrierEdwin K Jackson

Abstract

Although multiple biochemical pathways produce adenosine, studies suggest that the 2',3'-cAMP-adenosine pathway (2',3'-cAMP→2'-AMP/3'-AMP→adenosine) contributes to adenosine production in some cells/tissues/organs. To determine whether the 2',3'-cAMP-adenosine pathway exists in vivo in the brain, we delivered to the brain (gray matter and white matter separately) via the inflow perfusate of a microdialysis probe either 2',3'-cAMP, 3',5'-cAMP, 2'-AMP, 3'-AMP, or 5'-AMP and measured the recovered metabolites in the microdialysis outflow perfusate with mass spectrometry. In both gray and white matter, 2',3'-cAMP increased 2'-AMP, 3'-AMP and adenosine, and 3',5'-cAMP increased 5'-AMP and adenosine. In both brain regions, 2'-AMP, 3-AMP and 5'-AMP were converted to adenosine. Microdialysis experiments in 2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase) wild-type mice demonstrated that traumatic brain injury (controlled cortical impact model) activated the brain 2',3'-cAMP-adenosine pathway; similar experiments in CNPase knockout mice indicated that CNPase was involved in the metabolism of endogenous 2',3'-cAMP to 2'-AMP and to adenosine. In CSF from traumatic brain injury patients, 2',3'-cAMP was significantly increased in the i...Continue Reading

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Citations

Feb 8, 2014·Brain Research·Shlomit DachirEsther Shohami
Apr 9, 2014·Digestive Diseases and Sciences·Mervyn B FormanEdwin K Jackson
Sep 15, 2015·Brain Research·Matti MyllykoskiPetri Kursula
Jan 27, 2016·Journal of Neurochemistry·Edwin K JacksonPatrick M Kochanek
Nov 18, 2015·Journal of the American Society of Nephrology : JASN·Edwin K JacksonPatrick M Kochanek
Jun 14, 2013·The Journal of Pharmacology and Experimental Therapeutics·Edwin K Jackson, Zaichuan Mi
May 9, 2014·American Journal of Physiology. Renal Physiology·Edwin K JacksonPatrick M Kochanek
Mar 7, 2020·Journal of Neuroscience Research·Bruna Klippel FerreiraPatricia Fernanda Schuck
May 23, 2015·The Journal of Pharmacology and Experimental Therapeutics·Jonathan D VerrierEdwin K Jackson
Mar 16, 2017·Journal of Neurochemistry·Edwin K JacksonPatrick M Kochanek
Feb 23, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Edwin K JacksonPatrick M Kochanek
May 7, 2020·International Journal of Molecular Sciences·Krestinina OlgaPapadopoulos Vassilios
Jul 31, 2021·Biochemical and Biophysical Research Communications·Dongfang WangGuohua Cheng

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