The Brn-3a transcription factor induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins.

Molecular and Cellular Biology
M D SmithDavid S Latchman

Abstract

The Brn-3a POU family transcription factor is expressed only in posmitotic neurons in the central nervous system and identifies the first differentiated neurons to appear in the midbrain, hindbrain, and spinal cord during development. This factor is also induced when undifferentiated proliferating ND7 cells cease dividing and differentiate to a mature neuronal-like phenotype bearing numerous neurite processes. We show that overexpression of Brn-3a in undifferentiated ND7 cells induces a mature neuronal phenotype characterized by process outgrowth and the induction of genes encoding synaptic proteins, although the cells continue to proliferate. In contrast, the closely related factors Brn-3b and Brn-3c do not have this effect. Although the N-terminal activation domain of Brn-3a is required for maximum induction of neurite outgrowth and gene expression, these effects are primarily dependent on the DNA binding POU domain, which also acts as an activation domain. Overexpression of the isolated POU domain of Brn-3a is sufficient to induce neurite outgrowth, while the ability of full-length Brn-3a to do so is abolished by mutating a single amino acid in the Brn-3a POU homeodomain to its equivalent in Brn-3b. Thus, Brn-3a appears to p...Continue Reading

References

Sep 22, 1990·Proceedings. Biological Sciences·J N WoodM Theveniau
Jan 1, 1989·Annual Review of Neuroscience·J H Skene
Jun 30, 1995·The Journal of Biological Chemistry·N D LakinD S Latchman
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M R GerreroM G Rosenfeld
Jun 1, 1993·Annals of the Rheumatic Diseases·V B DhillonD S Latchman
Jun 1, 1993·Current Opinion in Cell Biology·M WegnerM G Rosenfeld
Apr 29, 1993·Biochimica Et Biophysica Acta·C P Verrijzer, P C Van der Vliet
Nov 1, 1995·Mechanisms of Development·N G Fedtsova, E E Turner
May 17, 1996·The Journal of Biological Chemistry·S J DawsonD S Latchman
Jan 1, 1996·Journal of Molecular Neuroscience : MN·Y Z LiuD S Latchman

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Citations

Mar 3, 2005·Neuroscience Research·H IchikawaT Sugimoto
May 6, 2004·Brain Research. Developmental Brain Research·H IchikawaT Sugimoto
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ji Ying Sze, Gary Ruvkun
May 19, 2004·Biochemical and Biophysical Research Communications·G R ThomasD S Latchman
Apr 13, 1999·Journal of Cellular Physiology·D S Latchman
Jun 15, 2004·Neuroreport·David J FaulkesDavid S Latchman
Apr 16, 1999·Endocrine Reviews·P L Dahia, A B Grossman
Mar 17, 2010·The Journal of Biological Chemistry·Daniel C BerwickDavid S Latchman
Apr 4, 2002·Neuroreport·Hiroyuki IchikawaTomosada Sugimoto
Aug 22, 1997·The Journal of Biological Chemistry·M D SmithD S Latchman
Jan 20, 2004·Molecular and Cellular Biology·R B Hough, J Piatigorsky
Feb 19, 2004·The Journal of Biological Chemistry·Shazia IrshadVishwanie Budhram-Mahadeo
Mar 29, 2001·Molecular and Cellular Neurosciences·M D SmithD S Latchman
Aug 25, 1997·Experimental Cell Research·A Lochter, M Schachner
Jan 21, 2000·Brain Research. Molecular Brain Research·M D SmithD S Latchman
Jan 14, 1998·Brain Research. Molecular Brain Research·Z C DeansD S Latchman
Jun 24, 1998·Brain Research. Molecular Brain Research·S WyattD S Latchman
Sep 13, 2002·Brain Research. Molecular Brain Research·H IchikawaT Sugimoto

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