The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)8-barrel-type seed proteins

Plant Physiology
A M. PerlickA Pühler

Abstract

Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis (alpha/beta)8-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of beta-sheets forming a central barrel surrounded by alpha-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.

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Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·S Janecek
May 18, 2000·Plant Science : an International Journal of Experimental Plant Biology·M FrühlingA M Perlick
May 11, 2004·Journal of Experimental Botany·Holger HesseRainer Hoefgen
Dec 19, 2009·Journal of Experimental Botany·Stefania AstolfiRainer Hoefgen
Jun 16, 2010·Journal of Experimental Botany·Yu WuCheng-Bin Xiang
Nov 18, 2011·Journal of Experimental Botany·S AstolfiR Pinton
Oct 8, 2009·Plant & Cell Physiology·Sharon PikeWalter Gassmann
Sep 1, 2006·Molecular Plant-microbe Interactions : MPMI·Nardjis AmiourEliane Dumas-Gaudot
Sep 24, 2014·Plant & Cell Physiology·María Del Carmen Rodríguez-HernándezMaría Del Carmen Martínez-Ballesta
Feb 9, 2010·Molecular Plant·Jean-Claude Davidian, Stanislav Kopriva
Jul 5, 2019·The Journal of Biological Chemistry·Süleyman GünalJonathan Wolf Mueller

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