The brome mosaic virus-based recombination vector triggers a limited gene silencing response depending on the orientation of the inserted sequence

Archives of Virology
Andrzej PacakMarek Figlerowicz

Abstract

In some RNA viruses (e.g. in brome mosaic virus, BMV), the same factor (intra- or intermolecular hybridization between viral RNA molecules) is capable of inducing two different processes: RNA silencing and RNA recombination. To determine whether there is some interplay between these two phenomena, we have examined if the BMV-based recombination vector containing a plant-genome-derived sequence can function as a gene-silencing vector. Surprisingly, we found that neither dsRNA forming during the replication of the BMV-based vector nor highly structured regions of its genome were effective RNAi triggers. Only mutants carrying a sequence complementary to the target mRNA functioned as gene silencing vectors and were steadily maintained in the infected plant. The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells.

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Citations

Dec 31, 2011·Cellular & Molecular Biology Letters·Adam KawalekWaclaw Orczyk
Nov 29, 2013·Plant Biotechnology Journal·Ming-Ru LiouYau-Heiu Hsu
May 26, 2017·Plant Cell Reports·Ravi Kant, Indranil Dasgupta
Jun 20, 2018·BMC Plant Biology·Dharmendra Kumar SinghKirankumar S Mysore

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