The Burkholderia cepacia fur gene: co-localization with omlA and absence of regulation by iron

Microbiology
C A LoweMark S Thomas

Abstract

The ferric uptake regulator (Fur) functions as a transcription repressor of many genes in bacteria in response to iron, but the presence of a functional equivalent of this protein has not been demonstrated in Burkholderia cepacia. A segment of the Burkholderia pseudomallei fur gene was amplified using degenerate primers and used to identify chromosomal restriction fragments containing the entire fur genes of B. cepacia and B. pseudomallei. These fragments were cloned and sequenced, revealing the Fur protein of both species to be a polypeptide of 142 amino acids possessing a high degree of amino acid sequence identity to Fur of other members of the beta subclass of the Proteobacteria. Primer extension analysis demonstrated that transcription of B. cepacia fur originated from a single promoter located 36 bp upstream from the fur translation initiation codon. The Fur polypeptide of B. cepacia was shown to functionally substitute for Fur in an Escherichia coli fur mutant. Single copy fur-lacZ fusions were constructed and used to examine the regulation of B. cepacia fur. The B. cepacia fur promoter was not responsive to iron availability, the presence of hydrogen peroxide or the superoxide generator methyl viologen. In addition, fur...Continue Reading

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Citations

Nov 8, 2002·Molecular Microbiology·Isabel DelanyVincenzo Scarlato
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·UNKNOWN Brazilian National Genome Project Consortium
Feb 14, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mark S Thomas
Aug 10, 2002·Journal of Bacteriology·Hiroyuki GenkaMasataka Tsuda

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