The Burkholderia thailandensis Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Burkholderia Myoviruses

Frontiers in Microbiology
Jens A HammerlStefan Hertwig

Abstract

Burkholderia mallei and B. pseudomallei are highly pathogenic species which are closely related, but diverse regarding their prophage content. While temperate phages have not yet been isolated from B. mallei, several phages of B. pseudomallei, and its non-pathogenic relative B. thailandensis have been described. In this study we isolated two phages from B. pseudomallei and three phages from B. thailandensis and determined their morphology, host range, and relationship. All five phages belong to the family Myoviridae, but some of them revealed different host specificities. DNA-DNA hybridization experiments indicated that the phages belong to two groups. One group, composed of ΦE058 (44,121 bp) and ΦE067 (43,649 bp), represents a new subgroup of Burkholderia myoviruses that is not related to known phages. The genomes of ΦE058 and ΦE067 are similar but also show some striking differences. Repressor proteins differ clearly allowing the phages to form plaques on hosts containing the respective other phage. The tail fiber proteins exhibited some minor deviations in the C-terminal region, which may account for the ability of ΦE058, but not ΦE067, to lyse B. mallei, B. pseudomallei, and B. thailandensis. In addition, the integrases and...Continue Reading

References

Sep 23, 1989·Lancet·N J WhiteN Pitakwatchara
May 1, 1989·The Journal of Infectious Diseases·W ChaowagulN Pitakwatchara
May 2, 1998·International Journal of Systematic Bacteriology·P J BrettD E Woods
Aug 15, 1998·Journal of Clinical Microbiology·A BauernfeindI Schneider
Mar 27, 1999·Journal of Bacteriology·A MatsushiroT Honda
Jun 11, 1999·Molecular Microbiology·M BarenboimR Young
Jul 28, 2001·The New England Journal of Medicine·A SrinivasanD L Thomas
Mar 19, 2002·Emerging Infectious Diseases·Lisa D RotzJames M Hughes
Jun 26, 2002·Journal of Bacteriology·Donald E WoodsDavid DeShazer
May 28, 2003·Lancet·N J White
Aug 6, 2003·Journal of Molecular Biology·Stefan HertwigBernd Appel
Jan 15, 2004·Emerging Infectious Diseases·Bart J Currie, Susan P Jacups
Jul 17, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·B GuerraM C Mendoza
Feb 21, 2006·International Journal of Food Microbiology·Dorothy NakimbugweChris W Michiels
Jun 8, 2007·Emerging Infectious Diseases·Wen-Chien KoYin-Ching Chuang
Aug 28, 2007·Current Opinion in Microbiology·Elizabeth J SummerRy Young
Dec 12, 2007·FEMS Microbiology Reviews·Waldemar Vollmer
Jul 5, 2008·Emerging Infectious Diseases·Sharon J PeacockBart J Currie
Nov 26, 2010·FEMS Microbiology Letters·Umaporn YordpratumRasana W Sermswan
Jul 28, 2011·Virology Journal·Jiraporn GatedeeSunee Korbsrisate
Apr 17, 2012·Applied and Environmental Microbiology·Omar Sepúlveda-RoblesGabriel Guarneros
Feb 19, 2013·Journal of Bacteriology·Dušanka Vesić, Christopher J Kristich
Sep 26, 2014·PLoS Neglected Tropical Diseases·Holger C ScholzUlrich Wernery
Dec 3, 2014·Frontiers in Microbiology·Jinyu ShanEdouard E Galyov
Dec 3, 2014·Journal of Structural Biology·Yingying ZhengRey-Ting Guo
Feb 10, 2016·Frontiers in Microbiology·Jens A HammerlStefan Hertwig
Sep 14, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Katherine A Rhodes, Herbert P Schweizer
Jul 18, 2017·Frontiers in Microbiology·Claudia JäckelJens A Hammerl
Jan 23, 2018·Journal of the European Academy of Dermatology and Venereology : JEADV·L FertittaE Caumes
Oct 20, 2018·Emerging Infectious Diseases·Jay E GeeAlex R Hoffmaster
May 10, 2019·BMC Bioinformatics·Debangana ChakravortySudipto Saha

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Datasets Mentioned

BETA
KT803877
MH809533
MH809535
PYIT00000000
PYIS00000000

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
electrophoresis
Electron microscopy

Software Mentioned

454 Newbler
Roche
454

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