The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica

Molecular Microbiology
Ming H YukJ F Miller

Abstract

The BvgAS signal transduction system in Bordetella spp. mediates a transition between infectious (Bvg+) and non-infectious (Bvg-) phases by sensing environmental conditions and regulating gene expression. Using differential display, arbitrary-primed polymerase chain reaction (PCR), we identified a gene expressed in the Bvg+ phase of Bordetella bronchiseptica that shows a high degree of sequence similarity to a locus involved in providing energy for type III secretion in pathogenic gram-negative bacteria (yscN in Yersinia spp.). We determined that the expression of this homologue in B. bronchiseptica (designated bscN) is regulated by bvg. Several open reading frames surrounding the bscN locus also show sequence similarity to loci encoding type III secretion apparatus components in other bacteria. An in-frame deletion of bscN in B. bronchiseptica leads to decreased secretion of several proteins, decreased cytotoxicity towards cultured cell lines and a defect in causing tyrosine dephosphorylation of specific proteins in infected cells in vitro. The deletion strain also revealed that bscN-mediated secretion is required for persistent colonization of the trachea in a rat infection model. Loci encoding type III secretion homologues w...Continue Reading

References

Jan 1, 1992·Current Topics in Microbiology and Immunology·C SasakawaH Watanabe
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D A RelmanS Falkow
Oct 1, 1989·Molecular Microbiology·G R CornelisJ C Vanooteghem
Apr 25, 1986·Nucleic Acids Research·C LochtJ M Keith
Apr 1, 1986·Journal of Bacteriology·J M MusserR K Selander
Dec 1, 1980·Microbiological Reviews·R A Goodnow
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Jul 1, 1994·Infection and Immunity·H SchipperR Gross
Sep 1, 1994·Infection and Immunity·K E Walker, A A Weiss
Feb 1, 1993·Microbial Pathogenesis·P H SavelkoulW Gaastra
Mar 1, 1994·Journal of Bacteriology·S WoestynG R Cornelis
Nov 25, 1993·Nucleic Acids Research·S A WoodsonS E Rokita
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A Uhl, J F Miller
Sep 11, 1993·Nucleic Acids Research·I M Khan, J M Coulson
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·R MénardC Dehio
Nov 29, 1996·Cell·E A Groisman, H Ochman
Jan 1, 1996·Annual Review of Cell and Developmental Biology·J E Galán, J B Bliska
Dec 1, 1996·Molecular Microbiology·T L YahrD W Frank
Mar 1, 1997·Molecular Microbiology·G R Cornelis, H Wolf-Watz

❮ Previous
Next ❯

Citations

Oct 24, 2003·Current Opinion in Microbiology·Mark J PallenIan R Henderson
Aug 29, 2000·Immunopharmacology·P A Cotter, J F Miller
Mar 12, 2003·Molecular and Cellular Probes·Katja StuberPeter Kuhnert
Apr 29, 2000·Trends in Microbiology·L W Cheng, O Schneewind
Jan 4, 2001·Microbes and Infection·L Gao, Y Abu Kwaik
Feb 19, 2000·Current Opinion in Microbiology·E T Harvill, J F Miller
Apr 22, 2004·Nature Reviews. Microbiology·Andrew PrestonDuncan J Maskell
Dec 21, 2000·Molecular Microbiology·K E StockbauerP A Cotter
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D M HeithoffM J Mahan
Aug 12, 2009·Infection and Immunity·Tracy L NicholsonSusan L Brockmeier
Jul 15, 2009·Infection and Immunity·Anne M BuboltzEric T Harvill
Jun 18, 2002·Infection and Immunity·Ulrich HeiningerEric T Harvill
Dec 24, 2002·Infection and Immunity·Valorie C BurnsEric T Harvill
May 23, 2003·Infection and Immunity·Bryna FuchslocherPeggy A Cotter
Aug 23, 2003·Infection and Immunity·Elizabeth J PishkoEric T Harvill
Oct 22, 2005·Infection and Immunity·Yolanda S López-BoadoRajendar Deora
Nov 22, 2005·Infection and Immunity·Paul B MannEric T Harvill
Sep 7, 2006·Journal of Clinical Microbiology·Paola StefanelliPaola Mastrantonio
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·S S Hirano, C D Upper
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Partho Ghosh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.