PMID: 6994881Jul 1, 1980Paper

The C-5 hydrogen isotope-effect in myo-inositol 1-phosphate synthase as evidence for the myo-inositol oxidation-pathway

Carbohydrate Research
M W Loewus, F A Loewus

Abstract

The hydrogen isotope-effect that occurs in vitro during myo-inositol1-phosphate synthase-catalyzed conversion of D-[5-3H]glucose 6-phosphate into myo[2-3H]inositol 1-phosphate has been used to compare the functional role of the nucleotide sugar oxidation-pathway with that of the myo-inositol oxidation-pathway in germinating lily pollen. Results reveal a significant difference between the 3H/14C ratios of glucosyl and galactosyluronic residues from pectinase-amyloglucosidase hydrolyzates of the 70% ethanol-insoluble fraction of D-[5-3H, 1-14C]glucose-labeled, germinating lily pollen. This isotope effect at C-5 of D-glucose that occurred during its conversion into D-galactosyluronic residues of pectic substance is not explained by loss of 3H when UDP-D-[5-3H, 1-14C]glucose is oxidized by UDP-D-glucose dehydrogenase from germinating lily pollen. The evidence obtained from this study favors a functional role for the myo-inositol oxidation-pathway during in vivo conversion of glucose into galactosyluronic residues of pectin in germinating lily pollen.

References

Sep 1, 1972·Archives of Biochemistry and Biophysics·M D Davies, D B Dickinson
Jun 1, 1978·Plant Physiology·A E Manthey, D B Dickinson

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Citations

Feb 1, 1983·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J F JacksonH F Linskens
Apr 12, 2005·Plant Molecular Biology·Laurence V BindschedlerG Paul Bolwell
Apr 3, 2009·Plant Physiology·Javad TorabinejadGlenda E Gillaspy
Sep 1, 1982·Plant Physiology·M W Loewus, F A Loewus
Jun 1, 1994·Burns : Journal of the International Society for Burn Injuries·J F TomeraS P Kukulka
Jun 24, 2017·Frontiers in Pharmacology·Maria A SortinoFilippo Drago
Jan 30, 2007·Molecular Microbiology·Abed N AzabMiriam L Greenberg
Sep 17, 2009·Biochemical Society Transactions·Rania M Deranieh, Miriam L Greenberg
Sep 24, 2020·Expert Opinion on Drug Metabolism & Toxicology·Fabio FacchinettiUNKNOWN Experts Group on Inositol in Basic and Clinical Research
Jan 10, 2002·The Journal of Biological Chemistry·Adam J Stein, James H Geiger
Nov 8, 2012·Organic & Biomolecular Chemistry·Rania M DeraniehMarie E Migaud

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