The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.

PLoS Genetics
Julien VandammeAnna Elisabetta Salcini

Abstract

Epigenetic modifications influence gene expression and provide a unique mechanism for fine-tuning cellular differentiation and development in multicellular organisms. Here we report on the biological functions of UTX-1, the Caenorhabditis elegans homologue of mammalian UTX, a histone demethylase specific for H3K27me2/3. We demonstrate that utx-1 is an essential gene that is required for correct embryonic and postembryonic development. Consistent with its homology to UTX, UTX-1 regulates global levels of H3K27me2/3 in C. elegans. Surprisingly, we found that the catalytic activity is not required for the developmental function of this protein. Biochemical analysis identified UTX-1 as a component of a complex that includes SET-16(MLL), and genetic analysis indicates that the defects associated with loss of UTX-1 are likely mediated by compromised SET-16/UTX-1 complex activity. Taken together, these results demonstrate that UTX-1 is required for many aspects of nematode development; but, unexpectedly, this function is independent of its enzymatic activity.

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Citations

Apr 29, 2015·Journal of Cellular Biochemistry·Di YangTatsuji Haneji
Mar 22, 2016·The Journal of Clinical Investigation·Hervé FaralliF Jeffrey Dilworth
Oct 18, 2015·Nucleic Acids Research·Julien VandammeAnna Elisabetta Salcini
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Nov 4, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Theodore VougiouklakisVassiliki Saloura
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Mar 27, 2021·BMC Biology·Abigail R R GuillermoAlison Woollard
Aug 21, 2020·Molecular and Cellular Biology·Nhien TranKai Ge

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Datasets Mentioned

BETA
U23513

Methods Mentioned

BETA
transgenic
PCR
Immunoprecipitation
Co-immunoprecipitations
size exclusion chromatography
gel filtration
genotyping
Fluorescence
pulldown
co-immunoprecipitation

Software Mentioned

SEQUEST
Xcalibur
Mascot
GraphPad
ImageJ
GraphPad Prism
Proteome Discoverer
MetaMorph
Adobe
Photoshop

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