PMID: 2496006Jan 1, 1989Paper

The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways

Genes & Development
A B ShyuJ G Belasco

Abstract

Rapid degradation of c-fos proto-oncogene mRNA is crucial for transient c-fos gene expression. Experiments were performed to investigate the cellular mechanisms responsible for the extremely short half-life of human c-fos mRNA in growth-factor-stimulated fibroblasts. These experiments demonstrate the existence of two distinct cellular pathways for rapid c-fos mRNA degradation. Each of these pathways recognizes a different, functionally independent instability determinant within the c-fos transcript. One instability determinant, which is located within the c-fos 3'-untranslated region, is a 75-nucleotide AU-rich segment. Insertion of this element into beta-globin mRNA markedly reduces the half-life of that normally long-lived message. Nevertheless, specific deletion of the AU-rich element from c-fos mRNA has little effect on the transcript's cytoplasmic half-life due to the presence of the other c-fos instability determinant, which is located in the protein-coding segment of the c-fos message. Examination of mRNA decay in cells treated with transcription inhibitors indicates that one c-fos mRNA degradation pathway is dependent on RNA synthesis, whereas the other is not.

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T Curran, J I Morgan
Jan 16, 1987·Cell·G Brawerman
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F MeijlinkI M Verma
Dec 10, 1988·Gene·J G Belasco, C F Higgins
Feb 25, 1987·Nucleic Acids Research·H J RahmsdorfP Herrlich
May 20, 1988·Science·F J RauscherB R Franza
Jan 20, 1985·Journal of Molecular Biology·A KrowczynskaG Brawerman
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A Maderious, S Chen-Kiang
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F van StraatenI M Verma
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R ZandomeniR Weinmann

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Citations

Jul 1, 1997·Journal of Cellular Biochemistry·B Gabbitas, E Canalis
Sep 10, 1998·Journal of Cellular Physiology·C H LeeV Ling
Feb 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I A Laird-Offringa
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SatoS I Murota
Mar 1, 1995·Journal of Cellular Physiology·G A Taylor, P J Blackshear
Dec 1, 1995·Journal of Cellular Physiology·T NakamakiH P Koeffler
Oct 1, 1996·Plant Molecular Biology·M L Abler, P J Green
Nov 27, 2004·Archives of Toxicology·Annamarie RogersD Clive Williams
Jun 1, 1995·Pharmacology & Therapeutics·K M McGowanP H Pekala
Apr 1, 1990·Molecular and Biochemical Parasitology·R M Coulson, D F Smith
Nov 11, 1991·Biochimica Et Biophysica Acta·M W Hentze
Apr 26, 1995·Biochimica Et Biophysica Acta·A G BlomqvistG J Martens
Jul 1, 1991·Virus Research·D C Henley, J P Weir
Mar 1, 1994·Brain Research. Molecular Brain Research·W J LukiwP St George Hyslop
Jul 1, 1996·Brain Research. Molecular Brain Research·P J Tolentino, L Villa-Komaroff
Jan 2, 1990·Molecular and Cellular Endocrinology·I A KlaijJ A Grootegoed
Feb 17, 1992·Neuroscience Letters·S S SchreiberM Baudry
Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Dec 16, 1992·Biochimica Et Biophysica Acta·S C SchiaviM E Greenberg
Jun 1, 1993·Free Radical Biology & Medicine·J A Melendez, C Baglioni
Sep 1, 1989·Trends in Biochemical Sciences·P Bernstein, J Ross
Nov 1, 2003·Archives of Biochemistry and Biophysics·Scott A MonkRobert H Rice

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