PMID: 9431802Feb 12, 1998Paper

The c-kit receptor is involved in the adhesion of mouse primordial germ cells to somatic cells in culture

Mechanisms of Development
M PesceMassimo De Felici

Abstract

The receptor encoded by the W (c-kit) locus is expressed on the membrane of mouse primordial germ cells, whereas its ligand termed stem cell factor (SCF), encoded by the Sl locus, is expressed on the membrane of somatic cells associated with both the primordial germ cell migratory pathways and homing sites. Using an in vitro short time assay which allows a quantitative measure of adhesion between cells, in the present paper we show that SCF/c-kit interaction can modulate primordial germ cell adhesion to somatic cells. We report that the adhesiveness of 11.5 dpc primordial germ cells to four types of somatic cells in culture (TM4 cells, STO fibroblasts, bone marrow stromal cells and gonadal somatic cells) is significantly reduced by antibodies directed against c-kit receptor or SCF, as well by soluble SCF. This SCF/c-kit mediated adhesion seems independent of SCF-induced tyrosine autophosphorylation of c-kit receptor. Moreover, primordial germ cells showed a poor ability to adhere to a bone marrow stromal cell line carrying the Sl(d) mutation (unable to synthesize membrane-bound SCF). This adhesiveness was not further impaired by anti-c-kit antibody. These results demonstrate that SCF/c-kit interaction contributes to the adhesio...Continue Reading

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Citations

Nov 20, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M PesceH R Schöler
Feb 15, 2001·Molecular Reproduction and Development·S FrankenbergL Selwood
Feb 8, 2011·In Vitro Cellular & Developmental Biology. Animal·Thomas J CapernaNeil C Talbot
Dec 6, 2003·The Journal of Allergy and Clinical Immunology·José M LoraJoshua A Boyce
Jan 30, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Patricia RodriguesDavid F Albertini
Jul 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Orly Lacham-Kaplan
Nov 13, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tamás OrdögKenton M Sanders
Dec 15, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Meina XieYuling Mi
Mar 10, 2001·Developmental Biology·N Matova, L Cooley
Oct 12, 2000·Developmental Biology·A Di Carlo, M De Felici
May 1, 2007·Developmental Biology·Donatella FariniMassimo De Felici
Oct 7, 2004·Gene Expression Patterns : GEP·James M SmithPeter Koopman
Nov 10, 2006·Biochemical and Biophysical Research Communications·Bindhu Paul-PrasanthYoshitaka Nagahama
Mar 20, 2010·Biology of Reproduction·Anja SchönerStephanie Schubert
Jul 7, 2007·Biology of Reproduction·Kazuhiro IwahashiYukiko Yamazaki
Aug 15, 2015·Genetics and Molecular Biology·Vahid MansouriAli Delbari
Mar 28, 2006·Nature Cell Biology·Paul W DyceJulang Li
Mar 31, 2006·The Journal of Reproduction and Development·Maki IshiiMasamichi Kurohmaru
Nov 2, 2017·Biology of Reproduction·Di ChenAmander T Clark
May 19, 2020·The Journal of Reproduction and Development·Maria SorrentiMassimo DE Felici

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